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Series GSE248099 Query DataSets for GSE248099
Status Public on Apr 04, 2024
Title Life-long persistence of nuclear RNAs in the mouse brain [NPCs]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Genomic DNA residing in the nuclei of mammalian neurons can be as old as the organism itself. The life span of non-coding nuclear RNAs, which are critical for proper chromatin architecture and transcription regulation, has not been determined in adult tissues. Here, we report the identification and characterization of nuclear RNAs that do not turn over for at least two years in the adult mouse brain. These long-lived RNAs (LL-RNA) are stably retained in nuclei in a neural cell type-specific manner and are required for the maintenance of heterochromatin. Thus, the remarkable life span of neural cells may depend on both the molecular longevity of DNA for the storage of genetic information but also the extreme stability of RNA for the functional organization of chromatin.
Overall design To identify long-retained RNAs in quiescent NPCs, in vitro NPCs were labelled with Ethynyluridine (EU) upon induction of quiescence and total RNA was isolated one week after EU administration. Controls were treated with PBS (vehicle) instead of EU. EU-labelled RNA was purified using Biotin-Click reaction and Streptavidin beads. To determine background binding, RNA from PBS-treated samples underwent the same pulldown procedure. Total RNA sequencing was performed using Universal Plus Total RNA-Sequencing kit (TECAN).
Contributor(s) Toda T
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Submission date Nov 17, 2023
Last update date Apr 04, 2024
Contact name Tomohisa Toda
Organization name DZNE
Street address Tatzberg 41
City Dresden
ZIP/Postal code 01307
Country Germany
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM7905883 NPCs, EU treatment, Rep1
GSM7905884 NPCs, EU treatment, Rep2
GSM7905885 NPCs, EU treatment, Rep3
This SubSeries is part of SuperSeries:
GSE248101 Life-long persistence of nuclear RNAs in the mouse brain
BioProject PRJNA1041774

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GSE248099_Count_table.xlsx 2.0 Mb (ftp)(http) XLSX
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