|
| Status |
Public on Apr 30, 2024 |
| Title |
ASXL1/2 links MLL4 and BAP1 on enhancers |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Histone H3K4 monomethyltransferases MLL3 and MLL4 contain a set of uncharacterized PHD fingers. By structural and biochemical assays, we found a novel function of the PHD2 and PHD3 (PHD2/3) fingers of MLL3 and MLL4, revealing their direct binding to the conserved MBH (MLL binding helix) region of ASXL1/2, components of the Polycomb repressive PR-DUB complex. In mouse embryonic stem cells, we observed that BAP1, the catalytic subunit of the PR-DUB complex, physically interacts with MLL4 in an ASXL1/2 MBH-dependent manner. Genomic studies demonstrate that the ASXL1/2 MBH is required for BAP1 binding on active enhancers and suggest that MLL4 facilitates BAP1 binding on active enhancers through ASXL1/2 MBH.
|
| |
|
| Overall design |
ChIP-Seq profiling of T7 tag and MLL4 as well as active enhancer marks H3K4me1 and H3K27ac in ESCs overexpressing BAP1-T7
|
| |
|
| Contributor(s) |
Guojia X, Ji-Eun L, Kai G |
| Citation(s) |
38849395 |
| |
| Submission date |
Nov 16, 2023 |
| Last update date |
Jul 30, 2024 |
| Contact name |
Kai Ge |
| E-mail(s) |
kai.ge@nih.gov
|
| Phone |
301-451-1998
|
| Organization name |
NIH
|
| Department |
NIDDK
|
| Street address |
50 South Dr Rm 4154
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA1041429 |
Less...
More...