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| Status |
Public on Apr 05, 2005 |
| Title |
Transcripts affected by RNAi to POLRMT |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Rho0 HeLa cells (devoid of mitochondrial DNA and functional mitochondria) were infected with recombinant lentiviral vector pLSLG expressing short hairpin RNAi complementary to Exons 3 and 17 of POLRMT gene. 48 hours after infection total RNA was isolated and analyzed for inhibition of POLRMT transcripts by RT-PCR. Total RNA from Rho0 HeLa infected with empty pLSLG vector was used as control. The two samples of RNA were used for microarray hybridization with Affymetrix human genome U133 2.0 plus microarray in order to monitor changes in gene expression patterns after inhibition of POLRMT gene.
Keywords: parallel sample
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| Contributor(s) |
Kravchenko JE, Rogozin IB, Koonin EV, Chumakov PM |
| Citation(s) |
16079853 |
| Submission date |
Apr 04, 2005 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Peter M Chumakov |
| E-mail(s) |
chumakp@ccf.org
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| Phone |
1-216-444-9540
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| Organization name |
Lerner Research Institute, The Cleveland Clinic Foundation
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| Department |
Molecular Genetics
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| Street address |
9500 Euclid Ave
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| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44195 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA91877 |
