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| Status |
Public on Nov 10, 2023 |
| Title |
The cullin Rtt101 promotes ubiquitin-dependent DNA-Protein Crosslink repair across the cell cycle |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
DNA‒protein crosslinks (DPCs) challenge faithful DNA replication and fluid passage of genomic information. Our study unveils the cullin ubiquitin ligase Rtt101 as a novel DPC repair factor. Genetic analyses demonstrate that Rtt101 is essential for resistance to a wide range of DPC types. Using an inducible in vivo DPC system, we reveal the significant impact of Rtt101 on DPC removal, including topoisomerase 1 crosslinks. ChIP-sequencing and ChEC-sequencing specifically highlight the association of Rtt101 with replisomes as well as colocalization with DPCs. Our findings establish Rtt101 as a novel main contributor to DPC repair throughout the yeast cell cycle.
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| Overall design |
Rtt101 ChEC-seq in absence (Raff) or presence (Gal) of a single Flp-Covalent Crosslink and colocalization of Rtt101 and the replication fork during S-phase by ChIP-seq.
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| Contributor(s) |
Noireterre A, Soudet J, Bagdiul I, Stutz F |
| Citation missing |
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| |
| Submission date |
Nov 10, 2023 |
| Last update date |
Nov 11, 2023 |
| Contact name |
Julien Soudet |
| E-mail(s) |
julien.soudet@unige.ch
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| Organization name |
Université de Genève
|
| Department |
BICEL
|
| Street address |
30 quai Ansermet
|
| City |
Geneva |
| ZIP/Postal code |
1204 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL27812 |
Illumina NovaSeq 6000 (Saccharomyces cerevisiae) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA1038787 |
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