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Series GSE247439 Query DataSets for GSE247439
Status Public on May 03, 2024
Title miRNA-mediated inhibition of an actomyosin network in hippocampal pyramidal neurons restricts sociability in adult male mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We recently reported that the loss of the microRNA cluster miR-379-410 leads to hypersocial behavior and anxiety in mice. With this study, we show that ablating miR-379-410 in excitatory neurons of the postnatal mouse hippocampus recapitulates hypersociability, but not anxiety. At the cellular level, miR-379-410 loss in excitatory neurons leads to increased excitatory synaptic transmission and upregulation of an actomyosin gene network. Re-expression of three cluster miRNAs, as well as pharmacological inhibition of the actomyosin activator ROCK, was sufficient to reinstate normal sociability in miR-379-410 knockout mice. Our results unveil a novel microRNA-actomyosin pathway involved in the control of sociability.
Overall design To investigate whether the knockout of miR379-410 miRNA cluster in glutamatergic neurons of the hippocampus drives differential gene expression, we established genetically modified mice with Cre-driven conditional knockout (cKO) of this cluster in Emx1 positive cells. We then performed gene expression profiling analysis using data obtained from RNA-seq of 6 different animals per genotype (6 wild type and 6 cKO mice).
Contributor(s) Narayanan R, Germain P, Schratt G
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Submission date Nov 09, 2023
Last update date May 03, 2024
Contact name Gerhard Schratt
Organization name ETH Zürich
Department D-HEST
Lab Systems Neuroscience
Street address Winterthurerstrasse 190
City Zürich
ZIP/Postal code 8057 Zürich
Country Switzerland
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM7889973 Wild type animal 1
GSM7889974 Wild type animal 2
GSM7889975 Wild type animal 3
BioProject PRJNA1037562

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