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Series GSE247398 Query DataSets for GSE247398
Status Public on Dec 06, 2023
Title Thrombocytes-derived Dickkopf1 promotes wound-healing macrophage polarization
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Immune responses are crucial to maintaining tissue homeostasis upon tissue injury. Upon various types of challenges, macrophages play a central role in regulating inflammation and tissue repair processes. While an immunomodulatory role of Wnt antagonist Dickkopf1 (DKK1) has been implicated, the role of Wnt antagonist DKK1 in regulating macrophage polarization in inflammation and the tissue repair process remains elusive. Here we found that DKK1 induces differential gene expression profiles from type 2-cytokine-activated macrophages to promote inflammation and tissue repair. Importantly, DKK1 induced pro-inflammatory and pro-resolving gene expressions via JNK (c-jun N-terminal kinase) in macrophages. Furthermore, DKK1 potentiated IL-13-mediated macrophage polarization and activation. Co-inhibition of JNK and STAT6 markedly decreased pro-inflammatory and pro-resolving gene expressions by DKK1 and IL-13. Interestingly, thrombocyte-specific deletion of DKK1 in mice reduced monocyte-derived macrophages in the acute sterile bleomycin (BLM)-induced lung injury model, suggesting that thrombocytes communicate with macrophages via DKK1 to orchestrate inflammation-induced injury repair process. Taken together, our study demonstrates DKK1’s role as a key regulatory role in macrophage polarization in the injury-induced inflammation and repair process.
 
Overall design Three biological replicates were analyzed for each of 4 conditions
BMDMs: untreated
BMDMs: IL-13 stimulation for 24 hr
BMDMs: DKK1 stimulation for 24 hr
BMDMs: IL-13+DKK1 stimulation for 24 hr
 
Contributor(s) Chae W, Park M, Sung E, Liu J, Alanya H, Erson-Omay EZ
Citation(s) 38162655
Submission date Nov 09, 2023
Last update date Jan 16, 2024
Contact name Eun-Ah Sung
E-mail(s) sungeunah92@gmail.com
Phone 2137326291
Organization name Virginia Common Wealth University
Street address 401 College Street
City Richmond
State/province VA
ZIP/Postal code 23298
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM7888922 WT_unstim_rep1
GSM7888923 WT_unstim_rep2
GSM7888924 WT_unstim_rep3
Relations
BioProject PRJNA1037482

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE247398_Macrophage_RNA_seq_processed_data.xlsx 1.7 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA

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