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Series GSE247343 Query DataSets for GSE247343
Status Public on May 10, 2024
Title Tyrosine phosphorylation of both STAT5A and STAT5B is necessary for maximal IL-2 signaling and its mitogenic action
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary STAT5 proteins are vital for lymphocyte development and function. Cytokine activation of STAT5A and STAT5B involves the tyrosine phosphorylation of a C-terminal tyrosine in each protein; however, the importance of STAT5 tyrosine phosphorylation in vivo has not been assessed. Here we generated Stat5a and Stat5b tyrosine mutant knockin mice and found they had greatly reduced CD8+ T-cell numbers. Morever, these cells exhibited profoundly diminished IL-2-induced proliferation, correlating with reduced IL-2- mediated induction of Myc, pRB, a range of cyclins and CDKs, and a G1àS phase- transition block. The mutant CD8+ T cells also exhibited decreased IL-2-mediated activation of pERK and pAKT, which can in part be attributed to diminished IL-2-induced expression of IL-2Rb and IL-2Rg. Our findings demonstrate that tyrosine phosphorylation of both STAT5A and STAT5B is essential for maximal IL-2 signaling, and our transcriptomic and proteomic analyses elucidate the molecular basis for the mitogenic effects of IL-2 on CD8+ T cells.
 
Overall design ChIP-Seq and RNA-Seq analyses using freshly isolated mouse CD8+ T cells that were expanded with either no cytokine, or with IL-2 at various time points.
 
Contributor(s) Lin J, Ge M, Liu C, Holewinski R, Andresson T, Gebregiorgis T, Li P, Leonard WJ
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Submission date Nov 08, 2023
Last update date May 11, 2024
Contact name Peng Li
E-mail(s) peng.li@nih.gov
Organization name NIH
Department NHLBI
Lab LMI
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (57)
GSM7887458 RNASeq STAT5A.0h.WT rep1
GSM7887459 RNASeq STAT5A.0h.WT rep2
GSM7887460 RNASeq STAT5A.0h.WT rep3
Relations
BioProject PRJNA1037130

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE247343_RAW.tar 1.4 Gb (http)(custom) TAR (of BW, RPKM)
SRA Run SelectorHelp
Raw data are available in SRA

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