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| Status |
Public on Nov 10, 2023 |
| Title |
Effect of Aurora-A knockdown on gene expression in murine CRC cell lines. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We aim to investigate the gene expression profile regulated by Aurora-A in murine CRC cell lines to identify genes that are either positively or negatively modulated by Aurora-A.
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| Overall design |
Aurora-A was knocked down in CT26 and MC38 cells using lentiviral-mediated short hairpin RNA (lenti-shmaurka-1). Cells transduced with lenti-shGFP served as the control group. At 48 hours post-seeding, the Aurora-A knockdown cells were harvested, and the RNA was extracted for comparative gene expression profiling using RNA-seq.
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| |
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| Contributor(s) |
Hung L, Lai M, Yang S |
| Citation(s) |
38291041 |
| |
| Submission date |
Nov 06, 2023 |
| Last update date |
Feb 12, 2024 |
| Contact name |
Shiang-Jie Yang |
| E-mail(s) |
s58074017@gs.ncku.edu.tw
|
| Organization name |
National Cheng Kung University
|
| Department |
Institute of Basic Medical Sciences
|
| Street address |
No.1, University Road, Tainan City 701, Taiwan (R.O.C)
|
| City |
Tainan |
| ZIP/Postal code |
701 |
| Country |
Taiwan |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1036129 |
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