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Series GSE246987 Query DataSets for GSE246987
Status Public on Aug 02, 2024
Title Mucosal Single-Cell Profiling of Crohn's-Like Disease of the Pouch Reveals Unique Pathogenesis and Therapeutic Targets
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Other
Summary The pathophysiology of Crohn’s-like disease of the pouch (CDP) that develops after restorative proctocolectomy with ileal pouch-anal anastomosis (IPAA) for ulcerative colitis (UC) is unknown. We examined mucosal cells from patients with and without CDP using single cell analyses.
 
Overall design Endoscopic samples were collected from the pouch body and pre-pouch ileum (refer to as “pouch/ileum”) of 50 patients with an IPAA. Single-cell RNA sequencing (scRNA-seq) was performed on pouch/ileal tissues of patients with normal pouch/ileum and CDP. Mass cytometry was performed on mucosal immune cells from patients with normal pouch/ileum, CDP, pouchitis, as well as those with familial adenomatous polyposis (FAP) after pouch formation. Findings were independently validated using immunohistochemistry.

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Submitter states that missing raw files are due to patient privacy concerns.
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Contributor(s) Cao S, Nguyen K, Colonna M
Citation(s) 39084267
Submission date Nov 03, 2023
Last update date Aug 03, 2024
Contact name Khai Nguyen
E-mail(s) k.m.nguyen@wustl.edu
Organization name Washington University in St. Louis
Department Pathology & Immunology
Lab Marco Colonna
Street address 425 South Euclid Ave, 8107, 8th floor BJCIH,
City Saint Louis
State/province MO
ZIP/Postal code 63112
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (59)
GSM7881808 pouch_ctrl1, scRNA-seq
GSM7881809 ileum_ctrl1, scRNA-seq
GSM7881810 pouch_ctrl2, scRNA-seq
Relations
BioProject PRJNA1035495

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE246987_RAW.tar 1.9 Gb (http)(custom) TAR (of CSV, MTX, TSV)
GSE246987_counts.txt.gz 373.4 Mb (ftp)(http) TXT
GSE246987_metadata.txt.gz 1.1 Mb (ftp)(http) TXT
Raw data not provided for this record

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