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Series GSE246748 Query DataSets for GSE246748
Status Public on Jul 17, 2024
Title CUT&Tag seq analysis of histone variant H2BE in neurons [H2BE_CT_antibodyValidation]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Analysis of histone variant H2BE in neuronal chromatin
 
Overall design WT and H2BE-KO primary cortical neurons were isolated from E16.5 mice and cultured for 12 days for validation of anti-H2BE antibody for CUT&Tag sequencing (n=1 biological replicates per genotype). KO serves as negative control to validate antibody.
 
Contributor(s) Feierman E
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 01, 2023
Last update date Jul 17, 2024
Contact name Emily Feierman
E-mail(s) emily.feierman@gmail.com
Organization name University of Pennsylvania
Street address 3400 Civic Center Blvd
City Philadelphia
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL21626 NextSeq 550 (Mus musculus)
Samples (3)
GSM7876340 primary cortical neurons, WT
GSM7876341 primary cortical neurons, H2BE-KO
GSM8239212 primary cortical neurons, WT, H3K27ac
This SubSeries is part of SuperSeries:
GSE246688 CUT&Tag, ATAC-seq, bulk RNA-seq and single-nucleus RNA-seq analysis of histone variant H2BE in cortical neurons
Relations
BioProject PRJNA1034551

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Supplementary file Size Download File type/resource
GSE246748_RAW.tar 241.9 Mb (http)(custom) TAR (of TDF)
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Raw data are available in SRA

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