GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE246551 Query DataSets for GSE246551
Status Public on Nov 02, 2023
Title Social isolation-induced transcriptomic changes in mouse hippocampus impact the synapse and show convergence with human genetic risk for neurodevelopmental phenotypes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Early life stress (ELS) can impact brain development and is a risk factor for neurodevelopmental disorders such as schizophrenia. Post-weaning social isolation (SI) is used to model ELS in animals, using isolation stress to disrupt a normal developmental trajectory. We aimed to investigate how SI affects the expression of genes in mouse hippocampus and to investigate how these changes related to the genetic basis of neurodevelopmental phenotypes. BL/6J mice were exposed to post-weaning SI (PD21-25) or treated as group-housed controls (n = 7-8 per group). RNA sequencing was performed on tissue samples from the hippocampus of adult male and female mice. Four hundred and 1,215 differentially-expressed genes (DEGs) at a false discovery rate of < 0.05 were detected between SI and control samples for males and females respectively. DEGS for both males and females were significantly overrepresented in gene ontologies related to synaptic structure and function, especially the post-synapse. DEGs were enriched for common variant (SNP) heritability in humans that contributes to risk of neuropsychiatric disorders (schizophrenia, bipolar disorder) and to cognitive function. DEGs were also enriched for genes harbouring rare de novo variants that contribute to autism spectrum disorder and other developmental disorders. Finally, cell type analysis revealed populations of hippocampal astrocytes that were enriched for DEGs, indicating effects in these cell types as well as neurons. Overall, these data suggest a convergence between genes dysregulated by the SI stressor in the mouse and genes associated with neurodevelopmental disorders and cognitive phenotypes in humans.
Overall design To investigate the effect of post-weaning social isolation on gene expression in the adult male and female mouse hippocampus tissue, we used RNA-seq to measure gene expression changes between socially-isolated (SI) animals and group-housed (GH) controls.
Web link
Contributor(s) Laighneach A, Kelly JP, Desbonnet L, Holleran L, Kerr DM, McKernan D, Donohoe G, Morris DW
Citation(s) 38127959
Submission date Oct 30, 2023
Last update date Feb 07, 2024
Contact name Aodan Laighneach
Organization name University of Galway
Street address University Rd.
City Galway
ZIP/Postal code H91 CF50
Country Ireland
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (30)
GSM7871153 Adult53-Hipp-A1, Female, SI
GSM7871154 Adult47-Hipp-B1, Female, GH
GSM7871155 Adult49-Hipp-C1, Female, SI
BioProject PRJNA1033618

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE246551_Raw_counts.txt.gz 973.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap