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Series GSE24631 Query DataSets for GSE24631
Status Public on Oct 04, 2011
Title Transcriptional failures arrest development after human nuclear transfer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Reprogramming occurs after nuclear transfer into zygotes whose genomes have been removed in mitosis, but not after nuclear transfer into zygotes enucleated in interphase. Our results suggest that there is a previously unappreciated barrier to successful human nuclear transfer, and that future studies should focus on the requirements for somatic genome activation.
 
Overall design 1-3 embryos were used for analysis. RNA amplification was done using two or three rounds of T7-mediated RNA amplification using the Illumina Total Prep RNA Amplification kit. Somatic cells 1-000 and 1-011 required only one round of RNA amplification because starting amounts of RNA were 100-500ng, while embryonic samples were amplified from single cells or embryos.
 
Contributor(s) Egli D, Eggan K
Citation(s) 21971503
Submission date Oct 12, 2010
Last update date Mar 20, 2017
Contact name Dieter Egli
E-mail(s) degli@nyscf.org
Phone 617 4968659
Organization name The New York Stem Cell Foundation Research Institute
Department NYSCF
Lab Egli
Street address 3960 Broadway
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (23)
GSM607458 human blastocyst, rep1
GSM607459 somatic donor cell 1-000, rep1
GSM607460 somatic donor cell 1-000, rep2
Relations
BioProject PRJNA132461

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24631_RAW.tar 3.9 Mb (http)(custom) TAR
GSE24631_non-normalized.txt.gz 3.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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