|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Oct 04, 2011 |
Title |
Transcriptional failures arrest development after human nuclear transfer |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
|
Summary |
Reprogramming occurs after nuclear transfer into zygotes whose genomes have been removed in mitosis, but not after nuclear transfer into zygotes enucleated in interphase. Our results suggest that there is a previously unappreciated barrier to successful human nuclear transfer, and that future studies should focus on the requirements for somatic genome activation.
|
|
|
Overall design |
1-3 embryos were used for analysis. RNA amplification was done using two or three rounds of T7-mediated RNA amplification using the Illumina Total Prep RNA Amplification kit. Somatic cells 1-000 and 1-011 required only one round of RNA amplification because starting amounts of RNA were 100-500ng, while embryonic samples were amplified from single cells or embryos.
|
|
|
Contributor(s) |
Egli D, Eggan K |
Citation(s) |
21971503 |
Submission date |
Oct 12, 2010 |
Last update date |
Mar 20, 2017 |
Contact name |
Dieter Egli |
E-mail(s) |
degli@nyscf.org
|
Phone |
617 4968659
|
Organization name |
The New York Stem Cell Foundation Research Institute
|
Department |
NYSCF
|
Lab |
Egli
|
Street address |
3960 Broadway
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
|
|
Platforms (1) |
GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
|
Samples (23)
|
GSM607461 |
amanitin-treated embryos, d5, rep1 |
GSM607462 |
amanitin-treated embryos, d5, rep2 |
GSM607463 |
human blastocyst, rep2 |
GSM607464 |
amanitin-treated embryos, d5, rep3 |
GSM607465 |
amanitin-treated embryos, d5, rep4 |
GSM607466 |
amanitin-treated embryos, d5, rep5 |
GSM607467 |
IVF control embryos d4, rep1 |
GSM607468 |
IVF control embryos d4, rep2 |
GSM607469 |
IVF control embryos d4, rep3 |
GSM607470 |
zygote |
GSM607471 |
zygote nuclear transfer embryo, rep1 |
GSM607472 |
somatic donor cell 1-011, rep1 |
GSM607473 |
somatic donor cell 1-011, rep2 |
GSM607474 |
amanitin-treated embryos, d3, rep1 |
GSM607475 |
amanitin-treated embryos, d3, rep2 |
GSM607476 |
zygote nuclear transfer embryo, rep2 |
GSM607477 |
IVF control embryos d3, rep1 |
GSM607478 |
IVF control embryos d3, rep2 |
GSM607479 |
abnormally cleaved embryo |
GSM607480 |
arrested 1-cell embryo |
|
Relations |
BioProject |
PRJNA132461 |
Supplementary file |
Size |
Download |
File type/resource |
GSE24631_RAW.tar |
3.9 Mb |
(http)(custom) |
TAR |
GSE24631_non-normalized.txt.gz |
3.5 Mb |
(ftp)(http) |
TXT |
Processed data included within Sample table |
|
|
|
|
|