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Series GSE245873 Query DataSets for GSE245873
Status Public on Apr 07, 2024
Title miRNA expression data from HPC-EVs
Platform organism synthetic construct
Sample organism Rattus norvegicus
Experiment type Non-coding RNA profiling by array
Summary EVs derived from HPCs could affect the progression of S. japonicum-induced fibrosis. Considering the EVs’ cargo, small RNAs account for a large proportion of cargoes in EVs, and act as one of the critical post-transcriptional regulators in cell-to-cell communication. Among them, miRNAs are the most studied and their regulatory roles in host–pathogen interactions are increasingly clear. HPCs are a kind of stem cells with potentially bidirectional differentiation ability and interact with hepatic stellate cells during liver injury. The activation of HPCs play an important role during S. japonicum-induced liver fibrosis.
We used microarrays to detail the gene expression of SEA-EVs and CON-EVs derived from HPC cell line, LE/6.
 
Overall design LE/6 was selected and cultured with soluble egg antigen (SEA) from S. japonicum or PBS for 24hours. Then cell culture medium from LE/6 was harvested and stored at -80℃ until isolation of EVs. Isolated EVs were collected to detect the miRNA expression.
 
Contributor(s) Yuan Y, Chen Q
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Submission date Oct 20, 2023
Last update date Apr 08, 2024
Contact name YUE YUAN
E-mail(s) D202181938@hust.edu.cn
Phone +8615521257192
Organization name tongji medical college
Street address none
City Wu Han
ZIP/Postal code 430000
Country China
 
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (6)
GSM7850161 EVs from PBS treated LE/6, biological rep1
GSM7850162 EVs from PBS treated LE/6, biological rep2
GSM7850163 EVs from PBS treated LE/6, biological rep3
Relations
BioProject PRJNA1030338

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Supplementary file Size Download File type/resource
GSE245873_RAW.tar 3.7 Mb (http)(custom) TAR (of CEL)

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