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| Status |
Public on Oct 17, 2023 |
| Title |
A cell atlas of the adult Aedes aegypti midgut revealed by single nucleus RNA-Seq |
| Organism |
Aedes aegypti |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mosquitoes are the most notorious hematophagous insects and due to their blood feeding behavior and genetic compatibility, numerous mosquito species are highly efficient vectors for certain human pathogenic parasites and viruses. The mosquito midgut is the principal organ of blood meal digestion and nutrient absorption. It is also the initial site of infection with blood meal acquired parasites and viruses. We conducted an analysis based on single-nucleus RNA sequencing(snRNA-Seq) to assess the cellular diversity of the midgut and how individual cells respond to blood meal ingestion to facilitate its digestion.
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| Overall design |
We dissected about 30 midguts from unfed and blood-fed Aedes aegypti mosquitoes, and isolated single nuclei, constructed two libraries, we analyzed the data with R package Seurat, the formed clusters were annotated. We further compared the cellular composition and gene expression between midguts from unfed and blood-fed mosquitoes.
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| Web link |
https://www.sciencedirect.com/science/article/pii/S0965174820301855#sec2
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| Contributor(s) |
Cui Y, Franz AW |
| Citation(s) |
33188922 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI134661 |
How Do Arboviruses Escape the Mosquito Midgut?- Analysis of a Novel Mechanism |
UNIVERSITY OF MISSOURI-COLUMBIA |
Alexander W E Franz |
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| Submission date |
Oct 17, 2023 |
| Last update date |
Oct 18, 2023 |
| Contact name |
Alexander W.E. Franz |
| E-mail(s) |
franza@missouri.edu
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| Organization name |
University of Missouri
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| Department |
Department of Veterinary Pathology
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| Street address |
303 Connaway Hall
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| City |
columbia |
| State/province |
MO |
| ZIP/Postal code |
65211 |
| Country |
USA |
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| Platforms (1) |
| GPL28657 |
Illumina NovaSeq 6000 (Aedes aegypti) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1029171 |
