GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE245596 Query DataSets for GSE245596
Status Public on Mar 18, 2024
Title Expression analysis of Plasmodium falciparum with MYST overexpression
Platform organism Plasmodium falciparum 3D7
Sample organism Plasmodium falciparum
Experiment type Expression profiling by array
Summary Investigation of genome-wide gene expression changes in Plasmodium falciparum overexpressing PfMYST compared to the wild-type strain 3D7.
After introducing a single copy of the full-length PfMYST expression cassette into the parasite genome, parasites showed higher levels of H4-K5, -K8, and -K12 acetylation, a faster progression of intraerythrocytic developmental cycle (IDC), and shorter schizont development time (duration), which led to significantly fewer merozoites developed in mature schizonts than the control. The parasites with PfMYST overexpression substantially increased the resistance to DNA-damaging agents. These findings were published in Miao, Fan et al Mol Microbiol 2010 (PMID: 20807207). To understand the underlying mechanisms, we studied changes in the transcriptomes during the IDC by expression microarray.
Overall design A 12 chip study using total RNA recovered from six time points (with 8h intervals from ring to schizont stage) of wild-type asexual cultures of Plasmodium falciparum 3D7 and six time points (with 8h intervals from ring to schizont stage) of asexual PfMYST-overexpression cultures. Each chip measures the expression level of 5,367 genes with 45-60 mer probes with two replicates on the array of 71618 probes.
Web link http://10.1128/msphere.00140-24
Contributor(s) Jun M
Citation(s) 38564734
Submission date Oct 17, 2023
Last update date Apr 05, 2024
Contact name JUN MIAO
Phone 8139747374
Organization name University of South Florida
Department Internal Medicine
Lab Jun Miao and Liwang Cui
Street address 3720 Spectrum Boulevard, MDC84
City Tampa
State/province Florida
ZIP/Postal code 33612
Country USA
Platforms (1)
GPL15768 NimbleGen P. falciparum 72K array [100712_Pfal_3D7_JM_exp_72K]
Samples (12)
GSM7845464 PF_wildtype_time point 1
GSM7845465 PF_wildtype_time point 2
GSM7845466 PF_wildtype_time point 3
BioProject PRJNA1029044

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE245596_RAW.tar 18.5 Mb (http)(custom) TAR (of PAIR)
GSE245596_RMA.txt.gz 265.8 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap