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Series GSE245161 Query DataSets for GSE245161
Status Public on May 01, 2024
Title Gene expression profile of Duocarmycin SA-treated human AML cell lines in vitro
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Acute myeloid leukemia (AML) is a hematological malignancy that is characterized by the expansion of immature myeloid precursors and poor patient survival outcomes. As such there is a need for the evaluation of potential therapeutic candidates that can be used in combination with current treatment regimens to improve patient outcomes. In this study, we investigated the efficacy of DSA on AML cells in vitro and performed gene expression profile analysis of DSA-treated AML cells to identify genes that are regulated by DSA. Here we report DSA-induced differentially expressed genes and cellular pathways that are associated with the functional changes that are observed in DSA-treated AML cells. Our data provides, for the first time, DSA's unique gene signature and provides a mechanistic rationale for DSA-induced functional effects in AML cells.
Overall design To determine the gene expression profile of DSA-treated human AML cells, human AML cell lines (Molm 14 and HL-60) were cultured with two different concentrations of DSA for 36 hours. At 36 hours, cells were harvested and RNA was isolated from the cells using Qiagen's RNeasy mini kit. RNA concentration and quality was determined using the nanodrop. Gene sequencing was performed by the Hartwell Center for Biotechnology (St. Jude Children's Research Hospital) and gene expression profiling analysis by the Center for Applied Bioinformatics (St. Jude Children's Research Hospital) and Loma Linda University.
Contributor(s) Francis-Boyle OL, Yang J, Fang J, Jin H, Chen WA, Boyle KE
Citation(s) 38673926
Submission date Oct 12, 2023
Last update date May 02, 2024
Contact name Hongjian Jin
E-mail(s) hongjian.jin@STJUDE.ORG
Organization name St Jude Children's Research Hospital
Department Center for Applied Bioinformatics
Street address 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38015
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM7837744 M14_ctrl_01
GSM7837745 M14_ctrl_02
GSM7837746 M14_DSA_Low_01
BioProject PRJNA1027284

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Supplementary file Size Download File type/resource
GSE245161_RAW.tar 19.6 Mb (http)(custom) TAR (of RESULTS)
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Raw data are available in SRA

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