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| Status |
Public on Nov 10, 2023 |
| Title |
dsRNA immunoprecipitation sequencing on a murine model of endotoxin-induced acute kidney injury |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
To investigate the extent of A-to-I RNA editing during endotoxemia in the kidney, we performed immunoprecipitation of dsRNA species, followed by stranded total RNA-seq on cytoplasmic and nuclear fractions of mouse kidneys.
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| Overall design |
C57BL/6J male mice were subjected to a single-dose, 4 mg/kg LPS (E. coli serotype 0111:B4) tail vein i.v. or vehicle saline in a volume of 300 ul. Kidney tissues were harvested at 0, 1, 4, 16, or 28 hours post-LPS injection, minced, and fractionated into cytoplasmic and nuclear compartments. Each fraction was immunoprecipitated using the anti-dsRNA monoclonal antibody J2 or IgG isotype control. cDNA library was prepared using the Clontech SMARTer Stranded Total RNA-Seq Kit v2.
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| Contributor(s) |
Hato T |
| Citation(s) |
38954486 |
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| Submission date |
Oct 10, 2023 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Takashi Hato |
| E-mail(s) |
thato@iu.edu
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| Phone |
317-278-4286
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| Organization name |
Indiana University
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| Department |
Medicine
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| Street address |
950 W. Walnut St. R2 202
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| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA1026469 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE244941_Sig_p_adj_FisherScoredSites_ByCondition_Cytoplasm_0hr_16hr.tsv.gz |
16.7 Kb |
(ftp)(http) |
TSV |
| GSE244941_Sig_p_adj_FisherScoredSites_ByCondition_Cytoplasm_0hr_1hr.tsv.gz |
616 b |
(ftp)(http) |
TSV |
| GSE244941_Sig_p_adj_FisherScoredSites_ByCondition_Cytoplasm_0hr_28hr.tsv.gz |
20.6 Kb |
(ftp)(http) |
TSV |
| GSE244941_Sig_p_adj_FisherScoredSites_ByCondition_Cytoplasm_0hr_4hr.tsv.gz |
1.3 Kb |
(ftp)(http) |
TSV |
| GSE244941_Sig_p_adj_FisherScoredSites_ByCondition_Nuclear_0hr_16hr.tsv.gz |
9.6 Kb |
(ftp)(http) |
TSV |
| GSE244941_Sig_p_adj_FisherScoredSites_ByCondition_Nuclear_0hr_1hr.tsv.gz |
757 b |
(ftp)(http) |
TSV |
| GSE244941_Sig_p_adj_FisherScoredSites_ByCondition_Nuclear_0hr_28hr.tsv.gz |
12.5 Kb |
(ftp)(http) |
TSV |
| GSE244941_Sig_p_adj_FisherScoredSites_ByCondition_Nuclear_0hr_4hr.tsv.gz |
1.7 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
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