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Series GSE244659 Query DataSets for GSE244659
Status Public on Mar 01, 2024
Title A diverse network of pericoerulear neurons control arousal states
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary As the primary source of norepinephrine (NE) in the brain, the locus coeruleus (LC) regulates both arousal and stress responses1,2. However, how local neuromodulatory inputs contribute to LC function remains unresolved. Here we identify a network of transcriptionally and functionally diverse GABAergic neurons in the LC dendritic field that integrate distant inputs and modulate modes of LC firing to control arousal. We define peri-LC anatomy using viral tracing and combine single-cell RNA sequencing and spatial transcriptomics to molecularly define both LC and peri-LC cell types. We identify several cell types which underlie peri-LC functional diversity using a series of complementary approaches in behaving mice. Our findings indicate that LC and peri-LC neurons comprise transcriptionally and functionally heterogenous neuronal populations, alongside anatomically segregated features which coordinate specific influences on behavioral arousal and avoidance states. Defining the molecular, cellular and functional diversity in the LC provides a road map for understanding the neurobiological basis of arousal alongside hyperarousal-related neuropsychiatric phenotypes.
Overall design six sections from female adult wild-type mouse and five sections from male adult mouse were used for spatial transcriptome analysis
Contributor(s) Luskin AT, Li L, Fu X, Barcomb K, Blackburn T, Li EM, Rana A, Simon RC, Sun L, Murry AD, Golden SA, Stuber GD, Ford CP, Gu L, Bruchas MR
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Submission date Oct 04, 2023
Last update date Mar 02, 2024
Contact name Xiaonan Fu
Organization name University of Washington
Department Biochemistry
Lab Gu
Street address 1705 NE Pacific St
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM7823491 female mouse, LC region
GSM7823492 male mouse, LC region
BioProject PRJNA1023977

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Supplementary file Size Download File type/resource
GSE244659_RAW.tar 26.7 Gb (http)(custom) TAR (of FA, RDS, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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