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| Status |
Public on Mar 01, 2024 |
| Title |
A diverse network of pericoerulear neurons control arousal states |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
As the primary source of norepinephrine (NE) in the brain, the locus coeruleus (LC) regulates both arousal and stress responses1,2. However, how local neuromodulatory inputs contribute to LC function remains unresolved. Here we identify a network of transcriptionally and functionally diverse GABAergic neurons in the LC dendritic field that integrate distant inputs and modulate modes of LC firing to control arousal. We define peri-LC anatomy using viral tracing and combine single-cell RNA sequencing and spatial transcriptomics to molecularly define both LC and peri-LC cell types. We identify several cell types which underlie peri-LC functional diversity using a series of complementary approaches in behaving mice. Our findings indicate that LC and peri-LC neurons comprise transcriptionally and functionally heterogenous neuronal populations, alongside anatomically segregated features which coordinate specific influences on behavioral arousal and avoidance states. Defining the molecular, cellular and functional diversity in the LC provides a road map for understanding the neurobiological basis of arousal alongside hyperarousal-related neuropsychiatric phenotypes.
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| Overall design |
six sections from female adult wild-type mouse and five sections from male adult mouse were used for spatial transcriptome analysis
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| Contributor(s) |
Luskin AT, Li L, Fu X, Barcomb K, Blackburn T, Li EM, Rana A, Simon RC, Sun L, Murry AD, Golden SA, Stuber GD, Ford CP, Gu L, Bruchas MR |
| Citation missing |
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| Submission date |
Oct 04, 2023 |
| Last update date |
Mar 02, 2024 |
| Contact name |
Xiaonan Fu |
| E-mail(s) |
xnfu@uw.edu
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| Organization name |
University of Washington
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| Department |
Biochemistry
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| Lab |
Gu
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| Street address |
1705 NE Pacific St
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1023977 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE244659_RAW.tar |
26.7 Gb |
(http)(custom) |
TAR (of FA, RDS, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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