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| Status |
Public on Apr 17, 2024 |
| Title |
An integrated toolkit for human microglia functional genomics |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Microglia, the brain’s resident immune cells, play vital roles in brain development, and disorders like Alzheimer’s disease (AD). Human iPSC-derived microglia (iMG) provide a promising model to study these processes. However, existing iMG generation protocols face challenges, such as prolonged differentiation time, lack of detailed characterization, and limited gene function investigation via CRISPR-Cas9.
In this study will generated iMG from human iPSCs using our newly developed protocol. We used single cell RNA-Seq to study the transcriptomic profiles of our newly formed iMG. The single cell RNA-Seq data under this accession is from wild type iMG (Hashtag 7) and OTHER KO iMG (Hashtag 8)
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| Overall design |
iPSC-derived microglia (iMG) were harvested from in vitro cultures, washed with PBS and hashtaged with hash tagging antibodies as: WT: Hashtag 7, OTHER KO Hashtag 8. scRNA-Seq was performed using 10X Genomics Chromium Next GEM single-cell 3' Reagent kit v3.1 leveraging its Feature Barcode technology.
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| Contributor(s) |
Haq I, Ngo JC, Menon V, Sher F |
| Citation(s) |
38600587 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AG070118 |
Functional dissection of fibronectin type 3 domains of SORL1 in Alzheimers disease associated microglia |
Trustees of Columbia University in the City of New York |
Falak Sher |
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| Submission date |
Oct 04, 2023 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Falak Sher |
| E-mail(s) |
fs2644@cumc.columbia.edu
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| Organization name |
Columbia University
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| Street address |
630 W 168th street
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1023967 |
