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Series GSE244602 Query DataSets for GSE244602
Status Public on Dec 01, 2023
Title A high-throughput screen identifies RNA Binding Proteins that affect fertility in C. elegans and reveals a functional relationship between ADR-2 and SQD-1
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary A high-throughput screen was performed to identify RNA binding proteins that impact fertility in Caenorhabditis elegans hermaphrodites. This screen makes use of animals expressing a vitellogenin (yolk protein) fusion with GFP, in a background (adr-2(-)) that corrects for a previously identified fertility defect in this strain. In addition to identifying several RNA binding proteins that regulate fertility, the screen also uncovered an interaction between the RNA binding proteins adr-2 and sqd-1, which were found to coregulate many transcripts important for fertility.
Overall design N2 (wildtype) and adr-2(-) animals were hatched and grown for 60 hours on ctrl (empty vector) RNAi or sqd-1 RNAi. Young adult worms were collected and total RNA was isolated from these samples. RNA was poly-A selected before proceeding with library preparation.
Contributor(s) Erdmann EA, Hundley HA
Citation(s) 37961348
Submission date Oct 04, 2023
Last update date Dec 01, 2023
Contact name Emily Ann Erdmann
Organization name Indiana University
Department Biology
Lab Hundley
Street address 1001 E Third Street
State/province IN
ZIP/Postal code 47405
Country USA
Platforms (1)
GPL32326 NextSeq 2000 (Caenorhabditis elegans)
Samples (12)
GSM7821578 N2 ctrl RNAi rep 1
GSM7821579 N2 ctrl RNAi rep 2
GSM7821580 N2 ctrl RNAi rep 3
BioProject PRJNA1023859

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Supplementary file Size Download File type/resource
GSE244602_N2_182_vs_adr2_182_deseq.csv.gz 1.9 Mb (ftp)(http) CSV
GSE244602_N2_ctrl_vs_N2_182_deseq.csv.gz 1.9 Mb (ftp)(http) CSV
GSE244602_N2_ctrl_vs_adr2_182_deseq.csv.gz 1.8 Mb (ftp)(http) CSV
GSE244602_N2_ctrl_vs_adr2_ctrl_deseq.csv.gz 1.7 Mb (ftp)(http) CSV
GSE244602_adr2_ctrl_vs_adr2_182_deseq.csv.gz 1.8 Mb (ftp)(http) CSV
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