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| Status |
Public on Mar 04, 2024 |
| Title |
BCL-2 modulates IRE1a activation to attenuate ER stress and pulmonary fibrosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
BCL-2 modulates IRE1a activation to attenuate ER stress and pulmonary fibrosis. Mice were treated with Bleomycin + Vehicle or Bleomycin + Navitoclax. Lungs were harvested 21 days after bleomycin injury followed by enzymatic digestion of the lungs to create single cell suspension and flow-sorting based isolation of total epithelial and stromal cells
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| Overall design |
Mice were treated with Bleomycin + Vehicle or Bleomycin + Navitoclax. Lungs were harvested 21 days after bleomycin injury followed by enzymatic digestion of the lungs to create single cell suspension and flow-sorting based isolation of total epithelial and stromal cells. The cells were then mixed in 1:1 ratio to perform single cell RNA-sequencing.
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| Contributor(s) |
Le Saux CJ, Ho T, Brumwell A, Kathiriya J, Wei Y, Auyeung V, Papa F, Chapman HA |
| Citation(s) |
38117250 |
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| Submission date |
Oct 03, 2023 |
| Last update date |
Mar 05, 2024 |
| Contact name |
Jaymin J Kathiriya |
| E-mail(s) |
jaymin.kathiriya@mssm.edu
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| Organization name |
University of California San Francisco
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| Department |
Medicine
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| Lab |
Chapman Lab
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| Street address |
513 Parnassus Ave
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94131 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1023585 |
