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| Status |
Public on Jul 05, 2024 |
| Title |
Short-term cold exposure induces persistent epigenomic memory in brown fat [ChIP-Seq |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Deficiency of the epigenome modulator histone deacetylase 3 (HDAC3) in brown adipose tissue (BAT) impairs the ability of mice to survive in near-freezing temperatures. Here, we report that short-term exposure to mild cold temperature (STEMCT: 15°C for 24 hours) averted lethal hypothermia of mice lacking HDAC3 in BAT (HDAC3 BAT KO) exposed to 4°C. STEMCT restored the induction of the thermogenic coactivator PGC-1a along with UCP1 at 22°C, which is greatly impaired in HDAC3-deficient BAT, and deletion of either UCP1 or PGC-1a prevented the protective effect of STEMCT. Remarkably, the protection of HDAC3 BAT KO mice from cold intolerance following STEMCT lasted for up to 7 days. Transcriptional activator C/EBPb was induced by short-term cold exposure in mouse and human BAT and, uniquely, remained high for 7 days following STEMCT. Furthermore, analysis of C/EBPb activity revealed increased binding following STEMCT at genes, including the enhancers/promotors of UCP1 and PGC-1a. These results reveal the existence of a cold-adaptive epigenomic memory mediated by C/EBPb that is persistent and HDAC3-independent.
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| Overall design |
Chromatin immunoprecipitation DNA sequencing for HDAC3, ERRalpha and C/EBPbeta in interscapular BAT of mice.
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| Contributor(s) |
Inoue S, Lazar MA, Midha M |
| Citation(s) |
38889724 |
| |
| Submission date |
Sep 29, 2023 |
| Last update date |
Oct 04, 2024 |
| Contact name |
Mohit K. Midha |
| Organization name |
University of Pennsylvania
|
| Street address |
3400 Civic Center Blvd
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE244386 |
Short-term cold exposure induces persistent epigenomic memory in brown fat |
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| Relations |
| BioProject |
PRJNA1022501 |
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