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Series GSE244380 Query DataSets for GSE244380
Status Public on Oct 15, 2023
Title Divergence of TORC1-mediated Stress Response Leads to Novel Acquired Stress Resistance in a Pathogenic Yeast
Organism Nakaseomyces glabratus
Experiment type Expression profiling by high throughput sequencing
Summary Acquired stress resistance (ASR) enables organisms to prepare for environmental changes that occur after an initial stressor. However, the genetic basis for ASR and how the underlying network evolved remain poorly understood. In this study, we discovered that a short phosphate starvation induces oxidative stress response (OSR) genes in the pathogenic yeast C. glabrata and protects it against a severe H2O2 stress; the same treatment, however, provides little benefit in the low pathogenic-potential relative, S. cerevisiae. This ASR involves the same transcription factors (TFs) as the OSR, but with different combinatorial logics. We show that Target-of- Rapamycin Complex 1 (TORC1) is differentially inhibited by phosphate starvation in the two species and contributes to the ASR via its proximal effector, Sch9. Therefore, evolution of the phosphate starvation-induced ASR involves the rewiring of TORC1’s response to phosphate limitation and the repurposing of TF-target gene networks for the OSR using new regulatory logics.
 
Overall design To test the hypothesis that phosphate starvation induces oxidative stress response genes in C. glabrata, conferring protection for H2O2 as a secondary stress, we profiled the transcriptional response at 1 hour of phosphate starvation in C. glabrata and compared it with the response to the same treatment in S. cerevisiae from (Zhou and O’Shea 2011). Two biological replicates before phosphate starvation and 3 biological replicates at 1 hour after the start of the starvation were collected and processed.
 
Contributor(s) He BZ
Citation(s) 37871123
NIH grant(s)
Grant ID Grant title Affiliation Name
R35 GM137831 Evolution of Stress Response Gene Regulatory Network in a Commensal and Opportunistic Yeast Pathogen THE UNIVERSITY OF IOWA Bin Z He
Submission date Sep 29, 2023
Last update date Jan 14, 2024
Contact name Bin He
E-mail(s) emptyhb@gmail.com, bin-he@uiowa.edu
Organization name the University of Iowa
Department Biology
Lab Gene Regulatory Evolution
Street address 129 East Jefferson St., Biology Building 450
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platforms (1)
GPL33801 Illumina HiSeq 4000 (Nakaseomyces glabratus)
Samples (5)
GSM7814516 before starvation, biol repl 1
GSM7814517 before starvation, biol repl 2
GSM7814518 1 hour after -Pi, biol repl 1
Relations
BioProject PRJNA1022497

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE244380_log2_norm_counts.txt.gz 209.2 Kb (ftp)(http) TXT
GSE244380_raw-counts.txt.gz 65.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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