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Status |
Public on Jan 11, 2024 |
Title |
Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [CITE-Seq] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The goal of this study was to examine the effect of dexamethasone-induced involution on the thymus cellular composition, and highlight cell populations which participate in the regeneration process. For this purpose we treated WT mice with dexamethasone, sorted non-T hematopoietic cells from their thymi 4 days after treatment when the thymus begins its recovery, or at an earlier time point (18 hours) for comparison, and performed CITE-Seq.
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Overall design |
Young WT male mice were treated with dexamethasone 4 days/18 hours before sacrifice, then perfused and stained with CITE-Seq antibodies. Thymic hematopoietic cells excluding mature T cells were then isolated by FACS sorting for 10x single cell RNA sequencing. 2 replicates were used for each condition. The control was untreated age-matched WT mice.
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Contributor(s) |
Abramson J, Nevo S, Leshkowitz D |
Citation(s) |
38215193 |
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Submission date |
Sep 28, 2023 |
Last update date |
Jan 15, 2024 |
Contact name |
Jakub Abramson |
E-mail(s) |
jakub.abramson@weizmann.ac.il
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Organization name |
Weizmann Institute of Science
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Department |
Department of Immunology
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Street address |
234 Herzl st.
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City |
Rehovot |
ZIP/Postal code |
7610001 |
Country |
Israel |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (6)
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GSM7809108 |
Thymic hematopoietic non-T cells, untreated1+18h dex2, GEX |
GSM7809109 |
Thymic hematopoietic non-T cells, untreated2+4d dex1, GEX |
GSM7809110 |
Thymic hematopoietic non-T cells, 18h Dex1+4d dex2, GEX |
GSM7809111 |
Thymic hematopoietic non-T cells, untreated1+18h dex2, Ab |
GSM7809112 |
Thymic hematopoietic non-T cells, untreated2+4d dex1, Ab |
GSM7809113 |
Thymic hematopoietic non-T cells, 18h Dex1+4d dex2, Ab |
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This SubSeries is part of SuperSeries: |
GSE244226 |
Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response |
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Relations |
BioProject |
PRJNA1021943 |