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Series GSE244229 Query DataSets for GSE244229
Status Public on Jan 11, 2024
Title Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [CITE-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study was to examine the effect of dexamethasone-induced involution on the thymus cellular composition, and highlight cell populations which participate in the regeneration process. For this purpose we treated WT mice with dexamethasone, sorted non-T hematopoietic cells from their thymi 4 days after treatment when the thymus begins its recovery, or at an earlier time point (18 hours) for comparison, and performed CITE-Seq.
 
Overall design Young WT male mice were treated with dexamethasone 4 days/18 hours before sacrifice, then perfused and stained with CITE-Seq antibodies. Thymic hematopoietic cells excluding mature T cells were then isolated by FACS sorting for 10x single cell RNA sequencing. 2 replicates were used for each condition. The control was untreated age-matched WT mice.
 
Contributor(s) Abramson J, Nevo S, Leshkowitz D
Citation(s) 38215193
Submission date Sep 28, 2023
Last update date Jan 15, 2024
Contact name Jakub Abramson
E-mail(s) jakub.abramson@weizmann.ac.il
Organization name Weizmann Institute of Science
Department Department of Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 7610001
Country Israel
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM7809108 Thymic hematopoietic non-T cells, untreated1+18h dex2, GEX
GSM7809109 Thymic hematopoietic non-T cells, untreated2+4d dex1, GEX
GSM7809110 Thymic hematopoietic non-T cells, 18h Dex1+4d dex2, GEX
This SubSeries is part of SuperSeries:
GSE244226 Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response
Relations
BioProject PRJNA1021943

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE244229_A_filtered_feature_bc_matrix.h5 34.0 Mb (ftp)(http) H5
GSE244229_B_filtered_feature_bc_matrix.h5 23.1 Mb (ftp)(http) H5
GSE244229_C_filtered_feature_bc_matrix.h5 37.9 Mb (ftp)(http) H5
GSE244229_general_filt_clust_20_pcs_1_2_res_SCT_average_expression.xlsx 4.8 Mb (ftp)(http) XLSX
GSE244229_mySeurat.rds.gz 997.6 Mb (ftp)(http) RDS
GSE244229_readme.txt 985 b (ftp)(http) TXT
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Raw data are available in SRA

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