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Status |
Public on Sep 29, 2023 |
Title |
Antiepileptic medication induces homeostatic synaptic plasticity in pyramidal neurons of the adult human neocortex |
Organisms |
Homo sapiens; Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Homeostatic synaptic plasticity serves to maintain neuronal function within a dynamic range, compensating for perturbations in network activity. While coordinated structural and functional changes at synaptic sites play a crucial role in adaptive processes, the specific regulatory mechanisms and biological relevance of homeostatic plasticity in the human brain warrant further investigation. In this study, we investigated the effects of neural network silencing, achieved through pharmacological inhibition of voltage-gated sodium channels or glutamatergic neurotransmission – common targets of antiepileptic medication – on functional and structural properties of murine and human cortical tissue. Using mouse entorhino-hippocampal tissue cultures, acute slices of adult mice, and human brain tissue, we characterize homeostatic synaptic plasticity across models, brain regions, and species. Our findings demonstrate local homeostatic synaptic plasticity in the adult human neocortex, highlighting the potential effects of antiepileptic medication in brain regions unaffected by the primary diseases, which might represent a mechanism for neuropsychiatric effects linked to these medications and increased seizure susceptibility upon discontinuation of antiepileptic medication.
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Overall design |
Comparative gene expression profiling analysis of RNA-seq data in different brain regions across species to decipher the effects of pharmacological activity deprivation in neural circuits.
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Contributor(s) |
Lenz M, Kruse P, Eichler A, Straehle J, Hemeling H, Stöhr P, Beck J, Vlachos A |
Citation missing |
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Submission date |
Sep 26, 2023 |
Last update date |
Sep 29, 2023 |
Contact name |
Maximilian Lenz |
E-mail(s) |
lenz.maximilian@mh-hannover.de
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Organization name |
Hannover Medical School
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Department |
Institute of Neuroanatomy and Cell Biology
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Street address |
Carl-Neuberg-Straße 1
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City |
Hannover |
ZIP/Postal code |
30625 |
Country |
Germany |
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Platforms (2) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (20)
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GSM7806572 |
Human Neocortex, AED-4, biol rep 4 |
GSM7806573 |
Human Neocortex, CONTROL-1, biol rep 1 |
GSM7806574 |
Mouse OTC, control-1, biol rep 1 |
GSM7806575 |
Mouse OTC, control-2, biol rep 2 |
GSM7806576 |
Mouse OTC, control-3, biol rep 3 |
GSM7806577 |
Mouse OTC, control-4, biol rep 4 |
GSM7806578 |
Mouse OTC, TTX-1, biol rep 1 |
GSM7806579 |
Mouse OTC, TTX-2, biol rep 2 |
GSM7806580 |
Mouse OTC, TTX-3, biol rep 3 |
GSM7806581 |
Mouse OTC, TTX-4, biol rep 4 |
GSM7806582 |
Mouse mPFC, CONTROL-1_biol rep 1 |
GSM7806583 |
Mouse mPFC, CONTROL-2_biol rep 2 |
GSM7806584 |
Mouse mPFC, CONTROL-3_biol rep 3 |
GSM7806585 |
Mouse mPFC, CONTROL-4_biol rep 4 |
GSM7806586 |
Mouse mPFC, LTG-1_biol rep 1 |
GSM7806587 |
Mouse mPFC, LTG-2_biol rep 2 |
GSM7806588 |
Mouse mPFC, LTG-3_biol rep 3 |
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Relations |
BioProject |
PRJNA1021222 |
Supplementary file |
Size |
Download |
File type/resource |
GSE244095_RAW.tar |
5.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
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