 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 31, 2024 |
| Title |
Chikungunya virus infection disrupts lymph node lymphatic endothelial cell composition and function via MARCO |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Infection with chikungunya virus (CHIKV) causes disruption of draining lymph node (dLN) organization, including paracortical relocalization of B cells, loss of the B cell-T cell border, and lymphocyte depletion that is associated with infiltration of the LN with inflammatory myeloid cells. Here, we find that during the first 24 h of infection, CHIKV RNA accumulates in MARCO-expressing lymphatic endothelial cells (LECs) in both the floor and medullary LN sinuses. The accumulation of viral RNA in the LN was associated with a switch to an antiviral and inflammatory gene expression program across LN stromal cells, and this inflammatory response, including recruitment of myeloid cells to the LN, was accelerated by CHIKV-MARCO interactions. As CHIKV infection progressed, both floor and medullary LECs diminished in number, suggesting further functional impairment of the LN by infection. Consistent with this idea, antigen acquisition by LECs, a key function of LN LECs during infection and immunization, was reduced during pathogenic CHIKV infection.
|
| |
|
| Overall design |
Identification and characterization of cells harboring CHIKV RNA using single-cell RNA sequencing.
|
| |
|
| Contributor(s) |
Lucas CJ, Sheridan RM, Hesselberth JR, Morrison TE |
| Citation(s) |
38194268 |
| |
| Submission date |
Sep 20, 2023 |
| Last update date |
May 03, 2024 |
| Contact name |
Ryan Sheridan |
| E-mail(s) |
ryan.sheridan@cuanschutz.edu
|
| Organization name |
University of Colorado Anschutz Medical Campus
|
| Street address |
12801 East 17th Avenue
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (14)
|
| GSM7792118 |
LN CD45- cells, chikv-2-8hpi |
| GSM7792119 |
LN CD45- cells, mock-1_enriched |
| GSM7792120 |
LN CD45- cells, mock-2_enriched |
| GSM7792121 |
LN CD45- cells, chikv-1-8hpi_enriched |
| GSM7792122 |
LN CD45- cells, chikv-2-8hpi_enriched |
| GSM7797748 |
LN, mock-1, 24hpi (re-analyzed) |
| GSM7797749 |
LN, mock-2, 24hpi (re-analyzed) |
| GSM7797750 |
LN, mock-3, 24hpi (re-analyzed) |
| GSM7797751 |
LN, chikv-1, 24hpi (re-analyzed) |
| GSM7797752 |
LN, chikv-2, 24hpi (re-analyzed) |
| GSM7797753 |
LN, chikv-3, 24hpi (re-analyzed) |
|
| Relations |
| BioProject |
PRJNA1019315 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE243638_RAW.tar |
188.4 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
| GSE243638_morrison_count_matrix.tsv.gz |
199.8 Mb |
(ftp)(http) |
TSV |
| GSE243638_morrison_metadata.tsv.gz |
4.8 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...