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Series GSE243076 Query DataSets for GSE243076
Status Public on Jan 11, 2024
Title Spatial transcriptomics and single-nucleus RNA-sequencing reveal a transcriptomic atlas of human spinal cord [Spinal snRNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Despite the recognized importance of the spinal cord in sensory processing, motor behaviors, and neural diseases, the underlying organization of neuronal clusters and their spatial location remain elusive. Recently, several studies have attempted to define the neuronal types and functional heterogeneity in the spinal cord using single-cell or single-nucleus RNA sequencing in animal models or developing humans. However, molecular evidence of cellular heterogeneity in the adult human spinal cord is limited. Here, we classified spinal cord neurons into 21 subclusters and determined their distribution from nine human donors using single-nucleus RNA sequencing and spatial transcriptomics. Moreover, we compared the human findings with previously published single-nucleus data of the mouse adult spinal cord, which revealed an overall similarity in the neuronal composition of the spinal cord between the two species while simultaneously highlighting some degree of heterogeneity. Additionally, we examined the sex differences in the spinal neuronal subclusters. Several genes, such as SCN10A and HCN1, showed sex differences in motor neurons. Finally, we classified human DRG neurons using spatial transcriptomics and explored the putative interactions between DRG and spinal cord neuronal subclusters. In summary, these results illustrate the complexity and diversity of spinal neurons in humans and provide an important resource for future research to explore the molecular mechanisms underlying spinal cord physiology and diseases.
Overall design Spatial transcriptomics and single-nucleus transcriptomic profiles of spinal cord in 9 human donors
Contributor(s) Zhang D, Chen Y, Wei Y, Chen H, Wu Y, Wu L, Li J, Miao C, Liu J, Zhu T, Zhou C
Citation(s) 38289829
Submission date Sep 13, 2023
Last update date Apr 02, 2024
Contact name Donghang Zhang
Organization name West China Hospital of Sichuan University
Street address No.37, guoxue lane, wuhou district, chengdu city, sichuan province
City Chengdu
State/province Sichuan
ZIP/Postal code 610041
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (9)
GSM7779044 Spinal_snRNA-seq_2
GSM7779045 Spinal_snRNA-seq_3
GSM7779046 Spinal_snRNA-seq_4
This SubSeries is part of SuperSeries:
GSE243077 Spatial transcriptomics and single-nucleus RNA-sequencing reveal a transcriptomic atlas of human spinal cord
BioProject PRJNA1016336

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Supplementary file Size Download File type/resource
GSE243076_RAW.tar 759.6 Mb (http)(custom) TAR (of TAR)
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Raw data are available in SRA

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