 |
 |
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Jan 11, 2024 |
Title |
Spatial transcriptomics and single-nucleus RNA-sequencing reveal a transcriptomic atlas of human spinal cord [Spinal spatial] |
Organism |
Homo sapiens |
Experiment type |
Other
|
Summary |
Despite the recognized importance of the spinal cord in sensory processing, motor behaviors, and neural diseases, the underlying organization of neuronal clusters and their spatial location remain elusive. Recently, several studies have attempted to define the neuronal types and functional heterogeneity in the spinal cord using single-cell or single-nucleus RNA sequencing in animal models or developing humans. However, molecular evidence of cellular heterogeneity in the adult human spinal cord is limited. Here, we classified spinal cord neurons into 21 subclusters and determined their distribution from nine human donors using single-nucleus RNA sequencing and spatial transcriptomics. Moreover, we compared the human findings with previously published single-nucleus data of the mouse adult spinal cord, which revealed an overall similarity in the neuronal composition of the spinal cord between the two species while simultaneously highlighting some degree of heterogeneity. Additionally, we examined the sex differences in the spinal neuronal subclusters. Several genes, such as SCN10A and HCN1, showed sex differences in motor neurons. Finally, we classified human DRG neurons using spatial transcriptomics and explored the putative interactions between DRG and spinal cord neuronal subclusters. In summary, these results illustrate the complexity and diversity of spinal neurons in humans and provide an important resource for future research to explore the molecular mechanisms underlying spinal cord physiology and diseases.
|
|
|
Overall design |
Spatial transcriptomics and single-nucleus transcriptomic profiles of spinal cord in 9 human donors
|
|
|
Contributor(s) |
Zhang D, Chen Y, Wei Y, Chen H, Wu Y, Wu L, Li J, Miao C, Liu J, Zhu T, Zhou C |
Citation(s) |
38289829 |
|
Submission date |
Sep 13, 2023 |
Last update date |
Apr 02, 2024 |
Contact name |
Donghang Zhang |
Organization name |
West China Hospital of Sichuan University
|
Street address |
No.37, guoxue lane, wuhou district, chengdu city, sichuan province
|
City |
Chengdu |
State/province |
Sichuan |
ZIP/Postal code |
610041 |
Country |
China |
|
|
Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
Samples (6)
|
|
This SubSeries is part of SuperSeries: |
GSE243077 |
Spatial transcriptomics and single-nucleus RNA-sequencing reveal a transcriptomic atlas of human spinal cord |
|
Relations |
BioProject |
PRJNA1016330 |
Supplementary file |
Size |
Download |
File type/resource |
GSE243074_RAW.tar |
399.2 Mb |
(http)(custom) |
TAR (of JPG, TAR) |
SRA Run Selector |
Raw data are available in SRA |
|
|
|
|
 |