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| Status |
Public on Jan 11, 2024 |
| Title |
Single-cell characterization of human conjunctiva tissue, organoids and air-liquid interface cultures (10X) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We mapped the cell types present in human conjunctiva tissue, organoids and air-liquid interface cultures exposed or not to IL4/13.
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| Overall design |
The following samples were subjected to single-cell mRNA sequencing: (1) human conjunctival organoids cultured in expansion medium for 5 days (donor 16 – palpebral – passage 5), (2) human conjunctival organoids cultured in expansion medium for 10 days and in differentiation medium for 7 days (donor 16 – palpebral – passage 4), (3) human conjunctival ALI when cells had just reached confluence (i.e. 3 days after seeding, named ALI day 0, from donor 16 – palpebral – passage 6), (4) human conjunctival ALI 3 days after shifting to air-liquid interface (donor 16 – palpebral – passage 5), (5) human conjunctival ALI 17 days after shifting to air-liquid interface (donor 16 – palpebral – passage 3, donor 4 – bulbar – passage 10), (6) human conjunctival ALI 15 days after shifting to air-liquid interface exposed to IL4/13 (donor 4 – bulbar – passage 4), and (7) human tissue from 4 pooled donors (all bulbar conjunctiva).
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| Web link |
https://doi.org/10.1016/j.stem.2023.12.008
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| Contributor(s) |
Bannier-Hélaouët M, Lamers M, Begthel H, Ma Z, Korving J, Giladi A, Begthel H, van de Wetering WJ, Lopez-Iglesias C, Yawata N, Dickman MM, LaPointe V, Kalmann R, Imhoff SM, Peters PJ, Wu W, Haagman B, Clevers H |
| Citation(s) |
38215738 |
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| Submission date |
Sep 05, 2023 |
| Last update date |
May 12, 2024 |
| Contact name |
Marie Bannier-Hélaouët |
| E-mail(s) |
m.bannier@hubrecht.eu
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| Organization name |
Hubrecht Institute
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| Street address |
Uppsalalaan 8
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| City |
Utrecht |
| ZIP/Postal code |
3584CT |
| Country |
Netherlands |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (4)
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| GSM7761925 |
Organoids EM, DM, and ALI day 0, day 3 and ALI + IL4/13 |
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| Relations |
| BioProject |
PRJNA1013334 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE242382_RAW.tar |
812.5 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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