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Series GSE242381 Query DataSets for GSE242381
Status Public on Apr 03, 2024
Title Hypothalamic thyroid hormone deficiency underlies reversible anorexia in a mammalian hibernator
Organism Ictidomys tridecemlineatus
Experiment type Expression profiling by high throughput sequencing
Summary Mammalian hibernators survive prolonged periods of cold and resource scarcity by temporarily modulating normal physiological functions, but the mechanisms underlying these adaptations are poorly understood. The hibernation cycle of thirteen-lined ground squirrels lasts for 5–7 months and comprises weeks of hypometabolic, hypothermic torpor interspersed with 24–48-hour periods of an active-like interbout arousal (IBA) state. We show that ground squirrels, who endure the entire hibernation season without food, have negligible hunger drive during IBAs. We further demonstrate that squirrels exhibit reversible inhibition of the hypothalamic feeding center, such that hypothalamic arcuate nucleus neurons have reduced sensitivity to the orexigenic and anorexigenic effects of ghrelin and leptin, respectively. However, hypothalamic infusion of thyroid hormone during an IBA is sufficient to rescue hibernation anorexia. Our results reveal that thyroid hormone deficiency underlies hibernation anorexia and demonstrate the functional flexibility of the hypothalamic feeding center.
Overall design Single cell gene expression in the arcuate nucleus of hypothalamus and median eminence was compared between active and hibernating thirteen-lined ground squirrels by single cell RNA sequencing on the 10X Genomics platform
Contributor(s) Mohr SM, Dai Pra R, Platt MP, Feketa VV, Shanabrough M, Varela L, Kristant A, Cao H, Merriman DK, Horvath TL, Bagriantsev SN, Gracheva EO
Citation(s) 38559054
Submission date Sep 05, 2023
Last update date Apr 04, 2024
Contact name Viktor Feketa
Organization name Yale University School of Medicine
Department Cellular & Molecular Physiology
Lab Elena Gracheva Lab
Street address 333 Cedar St SHM BE58
City New Haven
State/province Connecticut
ZIP/Postal code 06520
Country USA
Platforms (1)
GPL30340 Illumina NovaSeq 6000 (Ictidomys tridecemlineatus)
Samples (6)
GSM7761916 Active group, Subject 1
GSM7761917 Active group, Subject 2
GSM7761918 Active group, Subject 3
BioProject PRJNA1013335

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Supplementary file Size Download File type/resource
GSE242381_RAW.tar 894.6 Mb (http)(custom) TAR (of TAR)
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Raw data are available in SRA
Processed data provided as supplementary file

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