|
| Status |
Public on Sep 02, 2024 |
| Title |
Radial glia promote microglial development through aVb8 -TGFb1 signaling [ATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In this study, we aimed to identify 1) The relevant Itgb8 expressing cell types that mediate microglial TGFb activation; 2) The developmental timing of Itgb8-mediated TGFb signaling in microglia; 3) The cellular source and identity of the TGFb ligand relevant for microglial development and homeostasis; 4) The relationship between developmentally disrupted microglia and disease associated microglia; and 5) The role of canonical (Smad-mediated) versus non-canonical TGFb signaling in microglia.
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| |
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| Overall design |
ATAC-seq from Itgb8fl/fl; Emx1Cre microglia to determine open chromatin regions and comparison with transcriptional expression signature.
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| |
|
| Contributor(s) |
Mckinsey G, Santander N, Zhang X, Kilian K, Butovsky O, Arnold TD |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 01, 2023 |
| Last update date |
Sep 02, 2024 |
| Contact name |
Gabriel McKinsey |
| E-mail(s) |
gabriel.l.mckinsey@gmail.com
|
| Organization name |
University of California San Francisco
|
| Department |
Pediatrics
|
| Lab |
Arnold
|
| Street address |
1550 4th St. Room 545E
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94107 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (14)
|
| GSM7755821 |
Sample 4_ATA4, ITGB8KO |
| GSM7755822 |
Sample 5_ATA5, ITGB8KO |
| GSM7755823 |
Sample 6_ATA6, WT |
| GSM7755824 |
Sample 7_ATA7, ITGB8KO |
| GSM7755825 |
Sample 8_ATA8, WT |
| GSM7755826 |
Sample 9_ATA9, ITGB8KO |
| GSM7755827 |
Sample 10_ATA10, WT |
| GSM7755828 |
Sample 11_ATA11, ITGB8KO |
| GSM7755829 |
Sample 12_ATA12, WT |
| GSM7755830 |
Sample 13_ATA13, ITGB8KO |
| GSM7755831 |
Sample 14_ATA14, ITGB8KO |
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| This SubSeries is part of SuperSeries: |
| GSE242221 |
Radial glia promote microglial development through aVb8 -TGFb1 signaling |
|
| Relations |
| BioProject |
PRJNA1012104 |
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