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Series GSE241934 Query DataSets for GSE241934
Status Public on May 17, 2024
Title Neoadjuvant sintilimab plus chemotherapy in early-stage EGFR-mutant NSCLC: phase 2 trial interim results (NEOTIDE/CTONG2104)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Other
Summary We launched an investigator-initiated, Simon’s two-stage design trial of neoadjuvant sintilimab combined with carboplatin and nab-paclitaxel (nab-PC) in early-stage EGFR-mutant NSCLC (Clinicaltrial.gov number NCT05244213). Here we report the first interim results of stage 1 cohort which met the overall primary endpoint in advance, and multi-omics profiling of neoadjuvant immunotherapy combination in early-stage EGFR-mutant patients. We performed in-depth single-cell RNA/TCR sequencing (scRNA/TCR-seq) of cells derived from 11 resected tumors as well as 34 tumors from real-world cohort which were all confirmed wild-type lung adenocarcinoma (LUAD) or adeno-squamous carcinoma (ASC) and received neoadjuvant immunochemotherapy as control. By associating the tumor microenvironment (TME) and with responses, we uncovered heterogeneous mechanisms of primary resistance, providing insights into further strategic developments of combination regimens to improve the clinical outcome of EGFR-mutant NSCLC patients.
 
Overall design Non-erythrocytic living cells of 11 EGFR-mutant non-small cell lung cancer (NSCLC) and 34 wild-type lung adenocarcinoma (LUAD) or adeno-squamous carcinoma (ASC) that received neoadjuvant immunochemotherapy were isolated by gating 7AAD−CD235a− cells using Fluorescence-activated cell sorting (FACS), and analyzed using scRNA/TCR-seq.

>>>Submitter states that the raw data will be submitted to GSA.<<<
 
Contributor(s) Zhong W, Zhang C, Sun Y, Yi D, Jiang B
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Submission date Aug 30, 2023
Last update date May 18, 2024
Contact name Yuxuan Sun
E-mail(s) yuxuan.sun.1024@gmail.com
Organization name Peking University
Street address Yiheyuan Road
City Beijing
ZIP/Postal code 100871
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (88)
GSM7745818 scRNA-seq_P343
GSM7745819 scRNA-seq_P438
GSM7745820 scRNA-seq_P519
Relations
BioProject PRJNA1010782

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE241934_IIT_Matrix.mtx.gz 444.4 Mb (ftp)(http) MTX
GSE241934_IIT_Meta.txt.gz 1.9 Mb (ftp)(http) TXT
GSE241934_IIT_barcodes.tsv.gz 372.4 Kb (ftp)(http) TSV
GSE241934_IIT_features.tsv.gz 195.2 Kb (ftp)(http) TSV
GSE241934_RAW.tar 114.6 Mb (http)(custom) TAR (of CSV)
GSE241934_RWC_barcodes.tsv.gz 1.0 Mb (ftp)(http) TSV
GSE241934_RWC_features.tsv.gz 195.2 Kb (ftp)(http) TSV
GSE241934_Real_Matrix.mtx.gz 1.2 Gb (ftp)(http) MTX
GSE241934_Real_Meta.txt.gz 5.4 Mb (ftp)(http) TXT
Raw data not provided for this record
Processed data provided as supplementary file

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