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Series GSE241836 Query DataSets for GSE241836
Status Public on Sep 01, 2023
Title Analysis of the effect of prenatal alcohol exposure on the Placenta—Cortex transcriptomic signature
Organism Mus musculus
Experiment type Expression profiling by array
Summary Although alcohol consumption during pregnancy is a major cause of behavioral and learning disabilities, most FASD infants are late- or even misdiagnosed due to clinician’s difficulties achieving early detection of alcohol-induced neurodevelopmental impairments. Neuroplacentology has emerged as a new field of research focusing on the role of the placenta in fetal brain development. Several studies have reported that prenatal alcohol exposure (PAE) dysregulates a functional placenta–cortex axis, which is involved in the control of angiogenesis and leads to neurovascular-related defects.   However, these studies were focused on PlGF, a pro-angiogenic factor. The aim of the present study is to provide the first transcriptomic “placenta–cortex” signature of the effects of PAE on fetal angiogenesis. Whole mouse genome microarrays of paired placentas and cortices were performed to establish the transcriptomic inter-organ “placenta–cortex” signature in control and PAE groups at gestational day 20. Genespring   comparison of the control and PAE signatures revealed that 895 and 1501 genes were only detected in one of two placenta–cortex expression profiles, respectively. Gene ontology analysis indicated that 107 of these genes were associated with vascular development, and String protein–protein interaction analysis showed that they were associated with three functional clusters. PANTHER functional classification analysis indicated that “intercellular communication” was a significantly enriched biological process, and 27 genes were encoded for neuroactive ligand/receptors interactors.   Protein validation experiments involving Western blot for one ligand–receptor couple (Agt/AGTR1/2) confirmed the transcriptomic data, and Pearson statistical analysis of paired placentas and fetal cortices revealed a negative correlation between placental Atg and cortical AGTR1, which was significantly impacted by PAE. In humans, a comparison of a 38WG control placenta with a 36WG alcohol-exposed placenta revealed low Agt immunolabeling in the syncytiotrophoblast layer of the alcohol case. In conclusion, this study establishes the first transcriptomic placenta–cortex signature of a developing mouse. The data show that PAE markedly unbalances this inter-organ signature; in particular, several ligands and/or receptors involved in the control of angiogenesis. These data support that PAE modifies the existing communication between the two organs and opens new research avenues regarding the impact of placental dysfunction on the neurovascular development of fetuses. Such a signature would present a clinical value for early diagnosis of brain defects in FASD.
 
Overall design From gestational day (GD)15 to GD20, twelve pregnant mice received a daily subcutaneous injection of sodium chloride (NaCl 9‰) or alcohol (3g/kg, Fisher Scientific) diluted in NaCl (50%, v/v). At GD20, placentas and paired fetal cortices were collected, immediately frozen in liquid nitrogen and stored at −80 °C until use.
 
Contributor(s) Sautreuil C, Lecointre M, Derambure C, Brasse-Lagnel C, Leroux P, Laquerrière A, Nicolas G, Gil S, Savage DD, Marret S, Marguet F, Falluel-Morel A, Gonzalez BJ
Citation(s) 37686296
Submission date Aug 29, 2023
Last update date Sep 15, 2023
Contact name Anthony Falluel-Morel
Organization name Rouen University
Lab Inserm U1245
Street address 22 Boulevard Gambetta
City Rouen
ZIP/Postal code 76183
Country France
 
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (16)
GSM7744282 Cor-CT1-lame1
GSM7744283 Cor-CT2-lame2
GSM7744284 Cor-CT3-lame3
Relations
BioProject PRJNA1010216

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE241836_ALL_ENTITIES_PROCESSED.txt.gz 9.5 Mb (ftp)(http) TXT
GSE241836_RAW.tar 34.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data are available on Series record

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