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Series GSE241795 Query DataSets for GSE241795
Status Public on Nov 02, 2023
Title Effect of HSF1 overexpression in non-tumorigenic human breast epithelial MCF10A cell line
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To elucidate the mechanism of the epithelial-mesenchymal transition observed in MCF10A cell with HSF1 overexpression, we performed RNA-seq analyses. They revealed that cadherin signaling pathway (P00012) was the most enriched in the set of genes down-regulated in these cells. In addition to CDH1 and CDH2, 19 genes encoding protocadherins also showed reduced expression. Among the genes most severely affected in cells with HSF1 overexpression were many genes encoding cell adhesion or cytoskeleton-organizing proteins (e.g. GJA5, NID2, TUBA1A, MFAP5, TAGLN were inhibited, TTC25, CADM3, IQGAP2, SDK2, CEACAM6, FAT2 were upregulated).
Overall design To investigate the action of HSF1 in MCF10A cells, we established cells with stable HSF1 overexpression using lentiviruses. We then performed gene expression profilling using data from RNA-seq.
Contributor(s) Vydra N, Janus P, Stokowy T, Widłak W
Citation(s) 37894333
Submission date Aug 29, 2023
Last update date Nov 02, 2023
Contact name Tomasz Stokowy
Organization name University of Bergen
Department IT Division
Lab Scientific Computing
Street address Nygardsgaten 5
City Bergen
ZIP/Postal code 5020
Country Norway
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (4)
GSM7743898 MCF10A_WT_rep1
GSM7743899 MCF10A_HSF1_rep1
GSM7743900 MCF10A_WT_rep2
BioProject PRJNA1010099

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Supplementary file Size Download File type/resource
GSE241795_Counts_genocode_v26_MCF10A_GEO.txt.gz 5.0 Mb (ftp)(http) TXT
GSE241795_summary_table_RNA_MCF10A_GRCh38_GEO.txt.gz 500.6 Kb (ftp)(http) TXT
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