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| Status |
Public on Mar 31, 2024 |
| Title |
Gene expression profiles in rat alveolar macrophages exposure to cellulose nanofibrils, microcrystalline cellulose, and lipopolysaccharide. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
To assess the biological impact of cellulose nanofibrils (CNFs) at the transcriptional level, we conducted whole-genome microarray analyses on rat alveolar macrophages (NR8383) exposed to CNFs with varying physicochemical properties. The findings were compared with those from exposure to microcrystalline cellulose (MCC) and lipopolysaccharide (LPS).
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| Overall design |
Rat alveolar macrophages (NR8383) were exposed to six types of cellulose nanofibrils (CNFs: CNF1, CNF2, CNF3, CNF4, CNF5, and CNF6) at a concentration of 100 µg/mL, as well as MCC and LPS at concentrations of 100 µg/mL and 10 ng/mL, respectively, for 24 hours. The experiments included a vehicle control group (n=4). Cyanine-3-labeled cRNA, generated from NR8383 exposed to CNFs, MCC, and LPS was hybridized to a Whole Rat Genome Microarray. Subsequently, the scanned images of the hybridized microarray slides were subjected to numerical analysis to assess the transcriptional-level biological effects of CNFs on NR8383, compared with those from exposure to MCC and LPS.
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| Contributor(s) |
Fujita K, Obara S |
| Citation |
https://doi.org/10.1007/s10570-024-06238-4
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| Submission date |
Aug 28, 2023 |
| Last update date |
Nov 15, 2024 |
| Contact name |
Katsuhide Fujita |
| E-mail(s) |
ka-fujita@aist.go.jp
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| Organization name |
National Institute of Advanced Industrial Science and Technology
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| Department |
Research Institute of Science for Safety and Sustainability
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| Street address |
16-1 Onogawa
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| City |
Tsukuba |
| ZIP/Postal code |
3058569 |
| Country |
Japan |
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| Platforms (1) |
| GPL7294 |
Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Probe Name version) |
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| Samples (13)
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| GSM7743705 |
Rat alveolar macrophages, 24hr, Control for CNF1 and CNF3, 4 replicates |
| GSM7743706 |
Rat alveolar macrophages, 24hr, Control for CNF2 and CNF4, 4 replicates |
| GSM7743707 |
Rat alveolar macrophages, 24hr, Control for CNF5 and CNF6, 4 replicates |
| GSM7743708 |
Rat alveolar macrophages, 24hr, CNF1, 4 replicates |
| GSM7743709 |
Rat alveolar macrophages, 24hr, CNF2, 4 replicates |
| GSM7743710 |
Rat alveolar macrophages, 24hr, CNF3, 4 replicates |
| GSM7743711 |
Rat alveolar macrophages, 24hr, CNF4, 4 replicates |
| GSM7743712 |
Rat alveolar macrophages, 24hr, CNF5, 4 replicates |
| GSM7743713 |
Rat alveolar macrophages, 24hr, CNF6, 4 replicates |
| GSM7926378 |
Rat alveolar macrophages, 24hr, Control for MCC, replicate 4 |
| GSM7926379 |
Rat alveolar macrophages, 24hr, Control for LPS, replicate 4 |
| GSM7926380 |
Rat alveolar macrophages, 24hr, MCC, replicate 4 |
| GSM7926381 |
Rat alveolar macrophages, 24hr, LPS, replicate 4 |
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| Relations |
| BioProject |
PRJNA1009884 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE241791_RAW.tar |
468.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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