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Series GSE241444 Query DataSets for GSE241444
Status Public on Dec 13, 2023
Title XIST directly regulates X-linked and autosomal genes in naïve human pluripotent cells
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
Summary X-chromosome inactivation (XCI) serves as a paradigm for RNA-mediated regulation of gene expression, wherein the long non-coding RNA XIST spreads across the X-chromosome in cis to mediate chromosome-wide gene silencing. In female naïve human pluripotent stem cells (hPSCs), XIST is in a dispersed configuration and XCI does not occur, raising questions about XIST’s function. We found that XIST spreads across the X-chromosome and induces dampening of X-linked gene expression in naïve hPSCs. Surprisingly, XIST also targets specific autosomal regions, where it induces repressive chromatin changes and gene expression dampening. Thereby, XIST equalizes X-linked gene dosage between male and female cells while inducing differences on autosomes. The dispersed Xist configuration and autosomal localization also occur transiently during XCI initiation in mouse PSCs. Together, our study identifies XIST as the regulator of X-chromosome dampening, uncovers an evolutionarily conserved trans-acting role of XIST/Xist, and reveals a correlation between XIST/Xist dispersal and autosomal targeting.
 
Overall design Refer to individual Series
 
Citation(s) 38181737
Submission date Aug 22, 2023
Last update date Mar 13, 2024
Contact name Kathrin Plath
Organization name UCLA
Street address BOX 951737, 36-133 CHS
City Los Angeles
State/province CA
ZIP/Postal code 90095-1737
Country USA
 
Platforms (4)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (129)
GSM7728950 Early female naïve (UCLA1 pre XIST) R1
GSM7728951 Early female naïve (UCLA1 pre XIST) R2
GSM7728952 Female naïve ESCs (H9) Normoxia R1
This SuperSeries is composed of the following SubSeries:
GSE241438 XIST directly regulates X-linked and autosomal genes in naïve human pluripotent cells [RNA-seq]
GSE241439 XIST directly regulates X-linked and autosomal genes in naïve human pluripotent cells [RAP-seq_human]
GSE241440 XIST directly regulates X-linked and autosomal genes in naïve human pluripotent cells [RAP-seq_mouse]
Relations
BioProject PRJNA1008161

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Supplementary file Size Download File type/resource
GSE241444_RAW.tar 22.9 Mb (http)(custom) TAR (of BEDGRAPH, BROADPEAK)
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