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| Status |
Public on Aug 22, 2023 |
| Title |
Lactobacillus from the Altered Schaedler Flora maintain IFNγ homeostasis to promote behavioral stress resilience. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The gut microbiome consists of trillions of bacteria, fungi, and viruses that inhabit the digestive tract. These communities are sensitive to disruption from environmental exposures ranging from diet changes to illness. Disruption of the community of lactic acid producing bacteria, Lactobaccillacea, has been well documented in mood disorders and stress exposure. In fact, oral supplement with many Lactobacillus species can ameliorate these effects, preventing depression- and anxiety-like behavior. Here, we utilize a gnotobiotic mouse colonized with the Altered Schaedler Flora to remove the two native species of Lactobaccillacea. Using this novel microbial community, we found that the Lactobacillus species themselves, and not the disrupted microbial communities are protective from environmental stressors. Further, we determine that Lactobaccillacea are maintaining homeostatic IFNγ levels which are mediating these behavioral and circuit level responses. By utilizing the Altered Schaedler Flora, we have gained new insight into how probiotics influence behavior and provide novel methods to study potential therapies to treat mood disorders.
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| Overall design |
Microbiome comparative analysis via 16S sequencing of feces of C57Bl6J mice.
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| Contributor(s) |
Merchak AR, Wachamo S, Brown LC, Thakur A, Moreau B, Brown RM, Rivet-Noor C, Raghavan T, Gaultier A |
| Citation(s) |
37924959 |
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| Submission date |
Aug 17, 2023 |
| Last update date |
Nov 21, 2023 |
| Contact name |
Alban Gaultier |
| E-mail(s) |
ag7h@virginia.edu
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| Organization name |
University of Virginia
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| Street address |
409 Lane Rd
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| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22903 |
| Country |
USA |
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| Platforms (1) |
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| Samples (17)
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| GSM7716912 |
Feces, ex-germ free Naïve Fecese Recipient [Sample 57] |
| GSM7716913 |
Feces, ex-germ free Naïve Feces Recipient [Sample 58] |
| GSM7716914 |
Feces, ex-germ free Naïve Feces Recipient [Sample 59] |
| GSM7716915 |
Feces, ex-germ free Naïve Feces Recipient [Sample 60] |
| GSM7716916 |
Feces, ex-germ free Naïve Feces Recipient [Sample 61] |
| GSM7716917 |
Feces, ex-germ free Naïve Feces Recipient [Sample 62] |
| GSM7716918 |
Feces, ex-germ free Naïve Feces Recipient [Sample 63] |
| GSM7716919 |
Feces, ex-germ free Stress Feces Recipient [Sample 64] |
| GSM7716920 |
Feces, ex-germ free Stress Feces Recipient [Sample 65] |
| GSM7716921 |
Feces, ex-germ free Stress Feces Recipient [Sample 66] |
| GSM7716922 |
Feces, ex-germ free Stress Feces Recipient [Sample 67] |
| GSM7716923 |
Feces, ex-germ free Stress Feces Recipient [Sample 68] |
| GSM7716924 |
Feces, ex-germ free Stress Feces Recipient [Sample 69] |
| GSM7716925 |
Feces, SPF Naïve [Sample 70] |
| GSM7716926 |
Feces, SPF Naïve [Sample 71] |
| GSM7716927 |
Feces, SPF Stress [Sample 72] |
| GSM7716928 |
Feces, SPF Stress [Sample 73] |
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| Relations |
| BioProject |
PRJNA1006411 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE241110_ASV_Counts.csv.gz |
6.9 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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