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Series GSE240873 Query DataSets for GSE240873
Status Public on Dec 05, 2023
Title Glucocorticoid and Mineralocorticoid Receptor Signaling have Sex-dependent and Independent Effects in Mouse Hippocampus
Organism Mus musculus
Experiment type Expression profiling by array
Summary Many stress-related neuropsychiatric disorders display pronounced sex differences in their frequency and clinical symptoms. Glucocorticoids are primary stress hormones that have been implicated in the development of these disorders but whether they contribute to the observed sex bias is poorly understood. Glucocorticoids signal through two closely related nuclear receptors, the glucocorticoid (GR) and mineralocorticoid receptor (MR), that are both expressed at high levels in the hippocampus. To investigate the sex-dependent and independent actions of glucocorticoids in the hippocampus, we developed knockout mice lacking hippocampal GR (GREmx1-cre), MR (MREmx1-cre), or both GR and MR (GRMREmx1-cre). Genome-wide microarrays were performed on RNA isolated from the hippocampus of male and female flox control and knockout mice in order to 1) identify genes dysregulated in a sex-dependent and independent manner by a deficiency in GR, MR, or both GR and MR signaling and 2) identify the genes responsible for the sex-dependent and independent phenotypes observed in these mice.
Overall design Mice harboring a floxed GR allele and MR allele (dflox) and mice with conditional knockout of the glucocorticoid receptor (GR) (GREmx1-cre), mineralocorticoid receptor (MR) (MREmx1-cre), or both GR and MR (GRMREmx1-cre) in the hippocampus have been previously described (Oakley et al, Neurobiol Stress.2021 Jul 21;15:100369.doi: 10.1016/j.ynstr.2021.100369). For this microarray, total RNA was isolated from the whole hippocampus of adult male and female dflox control, GREmx1-cre, MREmx1-cre, and GRMREmx1-cre mice (n = 3-4 mice per genotype).
Contributor(s) Oakley RH, Cidlowski JA
Citation(s) 38075021
Submission date Aug 15, 2023
Last update date Dec 14, 2023
Contact name NIEHS Microarray Core
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (31)
GSM7711080 Male Hippocampus_dflox control_rep1
GSM7711081 Male Hippocampus_dflox control_rep2
GSM7711082 Male Hippocampus_dflox control_rep3
BioProject PRJNA1005616

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE240873_RAW.tar 68.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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