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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 20, 2023 |
| Title |
Homeostatic, repertoire and transcriptional relationships between colon T regulatory cell subsets |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Foxp3+ regulatory T cells (Tregs) in the colon are key to promoting peaceful co-existence with symbiotic microbes. Differentiated in either thymic or peripheral locations, and modulated by microbes and other cellular influencers, colonic Treg subsets have been identified through key transcription factors (TF; Helios, Rorg, Gata3, cMaf), but their inter-relationships are unclear. Here, we perform genomic analysis of colonic lamina propria Tregs with conditional KOs of each of these TFs to better understand how each TF contributes to colonic Treg identity and function.
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| Overall design |
Mouse CD4+ regulatory Tregs from WT or Treg-specific conditional TF KO (Gata3, Ikzf2, cMaf, Rorc) littermate control mice were isolated from colonic lamina propria by FACS for scATAC-seq using 10x Genomics.
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| Contributor(s) |
Ramanan D, Chowdhary K, Benoist C |
| Citation(s) |
38064511 |
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| Submission date |
Aug 11, 2023 |
| Last update date |
Dec 21, 2023 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
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| Phone |
617-432-7747
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| Organization name |
Harvard Medical School
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| Department |
Microbiology and Immunobiology
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| Lab |
CBDM
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA1004580 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE240657_RAW.tar |
2.1 Gb |
(http)(custom) |
TAR (of MTX, TBI, TSV, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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