NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE240515 Query DataSets for GSE240515
Status Public on Dec 15, 2023
Title Sex-specific epigenetics drive low GPER expression in gastrointestinal smooth muscles in Type 2 diabetic mice
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Type 2 diabetes mellitus (T2DM) causes gastroparesis, delayed intestinal transit, and constipation, for unknown reasons. Complications are predominant in men and postmenopausal women, suggesting a female hormone-mediated mechanism. Low G-protein coupled estrogen receptor (GPER) expression from epigenetic modifications may explain it. We explored sexually differentiated GPER expression and gastrointestinal symptoms related to GPER alterations in wild-type (WT) and T2DM mice (db/db). GPER mRNA and protein expression, DNA methylation and histone modifications were measured from stomach and colon samples of db/db and WT mice. We found that WT female tissues have the highest GPER mRNA and protein expressions. The expression is lowest in all db/db. The lowest mRNA levels are in the male stomach and female colon. GPER downregulation is associated with promoter hypermethylation and reduced enrichment of H3K4me3 and H3K27ac marks around the GPER promoter. T2DM impairs gut GPER expression, and epigenetic sex-specific mechanisms matter in the downregulation.
 
Overall design To investigate the GPER expression in male and female gastric and colonic smooth muscle of wild-type and db/db (type 2 diabetic) mice, we performed DNA-methylation measurement at the GPER promoter region by targeted bisulfite sequencing assay. This was performed in the colonic and gastric tissue of 5 male wild-type, 5 male db/db, 4 female wild-type, and 6 db/db female mice. The DNA methylation data was analyzed by comparative analysis, using ANOVA.
 
Contributor(s) Mahavadi S, Muhammad A
Citation(s) 38453938
Submission date Aug 09, 2023
Last update date Mar 13, 2024
Contact name Sunila Mahavadi
E-mail(s) smahavadi@tuskegee.edu
Phone 334-724-4366
Organization name Tuskegee University
Department Biology
Street address 1200 W. Montgomery Rd, Carver Research Building, Room #1
City Tuskegee
State/province Alabama
ZIP/Postal code 36088
Country USA
 
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (40)
GSM7699309 1WT-Male-1
GSM7699310 2WT-Male-2
GSM7699311 3WT-Male-3
Relations
BioProject PRJNA1003969

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE240515_raw_counts.txt.gz 3.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap