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Series GSE239798 Query DataSets for GSE239798
Status Public on Jan 25, 2024
Title Effect of Dyrk1a inhibition on the transcriptome of embryonic hearts from the Dp1Tyb mouse model of Down syndrome using bulk RNAseq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Embryos from the Dp1Tyb mouse model for Down syndrome (DS) present with congenital heart defects similar to the heart defects seen in humans with DS. We found that genetically reducing the copy number of the Dyrk1a gene (one of the genes in 3 copies in DS) from 3 to 2, normalised some of the transcriptomic changes in Dp1Tyb embryonic hearts and rescued congenital heart defects. Here we treated pregnant mice carrying Dp1Tyb and wild-type (WT) embryos with a Dyrk1a pharmacological inhibitor (Leucettinib-21 or L21) or an inactive isomer (Iso-L21) to study the effect of L21 on the transcriptome of Dp1Tyb and WT embryonic hearts.
Overall design C56BL/6J females were set up in timed matings with Dp1Tyb males and were treated daily by oral gavage with 30mg/kg of L21 or Iso-L21 from embryonic day 5.5 to E13.5. 20 embryonic hearts were used for this study; five biological repeats per condition (4 conditions: 2 genotypes treated with L21 or iso-L21) were used for bulk RNAseq.
Contributor(s) Lana-Elola E, Gilmore T, Tybulewicz V
Citation(s) 38266108
Submission date Aug 01, 2023
Last update date Apr 25, 2024
Contact name Victor Tybulewicz
Organization name Francis Crick Institute
Street address 1 Midland Road
City London
ZIP/Postal code NW1 1AT
Country United Kingdom
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (20)
GSM7673454 WT, IsoL21, 1
GSM7673455 WT, IsoL21, 2
GSM7673456 WT, IsoL21, 3
This SubSeries is part of SuperSeries:
GSE196447 Down Syndrome
BioProject PRJNA1000985

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Supplementary file Size Download File type/resource
GSE239798_RAW.tar 22.5 Mb (http)(custom) TAR (of RESULTS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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