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Series GSE239687 Query DataSets for GSE239687
Status Public on Dec 05, 2023
Title Genomic analysis of mice with endothelium-specific TERT knockout (scRNA-Seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Health of endothelial cells (EC) is fundamental for function of vital organs. However, it has not been established whether EC senescence underlies the pathophysiology of aging. Here, we report a mouse model of EC senescence. Inactivation of telomerase (TERT) expression sets the stage for aging and predisposes cells to replicative senescence. TERT maintains telomere length and has telomere-independent effects on cell transcriptome and physiology. We generated mice with TERT gene knock-out (KO) specifically in EC. We analyzed EC from adipose tissues (AT) and skeletal muscle cells from Tert-EC-KO and control mice raised on either chow or high-calorie diet (HCD) and subjected them to RNA sequencing (RNAseq). Single cell RNAseq data were also obtained for subcutaneous and visceral AT. Preliminary analysis of data deposited online indicates that TERT KO induces premature cell senescence and dysfunction. Moreover, HCD feeding exacerbates the effect of TERT loss. We conclude that the Tert-EC-KO mice are a useful model to study EC senescence and its effects on aging-associated organ dysfunction.
Overall design Tie2e-cre; TERT+/+; mTmG (WT) and Tie2e-cre; TERTfl/fl; mTmG (TERT-EC-KO) 7 month-old male mice were fed HFD for 5 months prior to cells isolation from subcutaneous AT (SC) and visceral intraperitoneal AT (IP).
Contributor(s) Kolonin M, Gao Z, Dai Y
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Submission date Jul 31, 2023
Last update date Dec 06, 2023
Contact name Yulin Dai
Organization name University of Texas health science center at Houston
Department School of Biomedical Informatics
Street address 7000 fannin, suite 600
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM7670006 WT SC AT scRNAseq
GSM7670007 WT IP AT scRNAseq
GSM7670008 EC-TERT-KO SC AT scRNAseq
BioProject PRJNA1000567

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Supplementary file Size Download File type/resource
GSE239687_RAW.tar 40.4 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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