|
| Status |
Public on Aug 01, 2024 |
| Title |
Transcription factor KLF2 regulates cell fate decision of stem cells in infantile hemangioma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
As the most common vascular tumor during infancy, infantile hemangioma (IH) is clinically featured by a rapid proliferation phase of disorganized blood vessels and a subsequent spontaneous involution phase. Infantile hemangioma arises from a unique type of multipotent stem cells called hemangioma stem cells (HemSCs), which could differentiate into endothelial cells, pericytes and adipocytes in IH. However, the underlying mechanisms that regulate the cell fate determination of HemSCs are not well elucidated. Here, we identified KLF2 as a candidate transcription factor involved in the control of HemSCs differentiation. KLF2 was expressed in endothelial cells in proliferating IH and its expression diminished in adipocytes in involuting IH. KLF2 regualtes the proliferation, apopotosis and cell cycle progression in HemSCs. Moreover, KLF2 is a critical regulator in HemSCs that control their differentiation direction between endothelial cells and adipocytes. Knockdown of KLF2 inhibited the formation of blood vessels in vivo while accelerated the progress of adipogenesis. RNA-seq analysis suggested an induction of pro-adipogenic transcriptome in HemSCs upon KLF2 knockdown. Our data showed that KLF2 exhibited pleiotropic effects in regulating the biological behaviours of HemSCs, and was involved in the progression and involution of IH via determining the cell fate of HemSCs.
|
| |
|
| Overall design |
In this study ,we performed RNA-seq in KLF2 knockdown HemSCs (shKLF2) and control HemSCs (shControl) to dissect the transcriptional alterations in response to KLF2.
|
| |
|
| Contributor(s) |
Zheng J, Bian Q, Chen Q |
| Citation(s) |
38382868 |
| |
| Submission date |
Jul 26, 2023 |
| Last update date |
Oct 31, 2024 |
| Contact name |
Qian Bian |
| E-mail(s) |
qianbian@shsmu.edu.cn
|
| Phone |
86-21-3845-2656
|
| Organization name |
Shanghai Jiao Tong University School of Medicine
|
| Department |
Shanghai Institute of Precision Medicine, Ninth People’s Hospital
|
| Street address |
Jinzun Road No.115
|
| City |
Shanghai |
| ZIP/Postal code |
200011 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA998877 |
Less...
More...