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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 29, 2024 |
| Title |
Utilization of Tagged Transgenic Mouse Lines to Study the Molecular Roles of SMAD1/5 in Mediating Signaling Crosstalk During Early Pregnancy II |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In this study, we generated two novel transgenic mouse lines with HA- or PA- affinity tags in the SMAD1 and SMAD5 loci (Smad1HA/HA and Smad5PA/PA) to define how these proteins integrate BMP signaling in the uterus during early pregnancy. Using CUT & RUN, we provided genomic evidence demonstrating the unique and shared roles of SMAD1 and SMAD5 during the window of implantation.
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| Overall design |
We performed Cleavage Under Targets & Release Using Nuclease (CUT&RUN-seq) for SMAD1 and SMAD5 proteins in the mouse mouse uterus at 4.5 days post coitus timepoint.
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| Contributor(s) |
Liao Z, Tang S, Nozawa K, Shimada K, Ikawa M, Monsivais D, Matzuk MM |
| Citation(s) |
38536963 |
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| Submission date |
Jul 21, 2023 |
| Last update date |
Apr 04, 2024 |
| Contact name |
Martin M Matzuk |
| E-mail(s) |
mmatzuk@bcm.edu
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| Organization name |
Baylor College of Medicine
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| Department |
Pathology and Immunolodgy
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| Lab |
Matzuk
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| Street address |
One Baylor Plaza
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (10)
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| GSM7657198 |
Uterus,SMAD5-KI,IgG,rep1 |
| GSM7657199 |
Uterus,SMAD5-KI,PA,rep1 |
| GSM7657200 |
Uterus,SMAD1-KI,IgG,rep2 |
| GSM7657201 |
Uterus,SMAD1-KI,HA,rep2 |
| GSM7657202 |
Uterus,SMAD1-KI,PR,rep2 |
| GSM7657203 |
Uterus,SMAD5-KI,IgG,rep2 |
| GSM7657204 |
Uterus,SMAD5-KI,PA,rep2 |
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| Relations |
| BioProject |
PRJNA997324 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE237975_RAW.tar |
2.0 Gb |
(http)(custom) |
TAR (of BED, BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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