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Series GSE237829 Query DataSets for GSE237829
Status Public on Jul 25, 2023
Title Flexible and scalable control of T cell memory by a reversible epigenetic switch (sci-fate-seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In this study, we use a minimal ex vivo culture system to track CD8 T cell differentiation at the single cell and clonal lineage level during activation. To characterize early heterogeneity in memory and effector differentiation in this system, we subjected cells to temporally-resolved single-cell transcriptomic profiling using sci-fate-seq (Cao et al., Nature Biotech, 202).
Overall design Cells were activated ex vivo and subjected at days 1, 2, and 4 to 4-thiouridine (4sU) pulse-labeling for 2 hrs, enabling detection of newly synthesized mRNA at each time point.
Contributor(s) Abadie K, Yang W, Cao J, Shendure J, Kueh H
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Submission date Jul 20, 2023
Last update date Jul 25, 2023
Contact name Kathleen Abadie
Organization name University of Washington
Department Bioengineering
Lab Kueh Lab
Street address 3720 15th Ave NE
City Seattle
State/province Washington
ZIP/Postal code 98195
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (1)
GSM7655063 T cell sci-fate
This SubSeries is part of SuperSeries:
GSE237830 Flexible and scalable control of T cell memory by a reversible epigenetic switch
BioProject PRJNA996895

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Supplementary file Size Download File type/resource
GSE237829_RAW.tar 209.9 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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