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Series GSE237770 Query DataSets for GSE237770
Status Public on Apr 17, 2024
Title Coordination of histone chaperones for parental histone segregation and epigenetic inheritance
Organism Schizosaccharomyces pombe
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chromatin-based epigenetic memory relies on the accurate distribution of parental histone tetramers to newly replicated DNA strands, serving as templates for chromatin structure duplication. Mcm2, a subunit of the replicative helicase, and the Dpb3/4, subunits of polymerase epsilon, govern parental histone deposition to the lagging and leading strands, respectively. However, their contribution to epigenetic inheritance remains controversial. Here we show in fission yeast that a Mcm2 histone chaperone mutation severely disrupts heterochromatin inheritance, while Dpb3/4 mutations cause only moderate defects. Surprisingly, simultaneous Mcm2 and Dpb3/4 mutations stabilizes heterochromatin inheritance. eSPAN analyses confirm the conservation of Mcm2 and Dpb3/4 functions in parental histone segregation, with their collective absence reducing segregation bias. Furthermore, the FACT histone chaperone also regulates parental histone transfer independently of strands and collaborates with Mcm2 and Dpb3/4 to maintain parental histone density, ensuring faithful heterochromatin inheritance. These results underscore the importance of precise parental histone segregation to the lagging strand for epigenetic inheritance and unveil unique properties of parental histone chaperones during DNA replication.
Overall design Input, BrdU, H3K56ac ChIP-Seq, H3K56ac eSPAN, H3K4me3 ChIP-Seq, H3K4me3 eSPAN, are performed in S.pombe followed by Next Generation Sequencing
Contributor(s) Fang Y, Hua X, Zhang Z, Jia S
Citation(s) 38479839
Submission date Jul 19, 2023
Last update date Apr 18, 2024
Contact name zhiguo zhang
Organization name Columbia University
Department Pediatric and Genetics and Development
Lab Irving Cancer Research Center
Street address 1130 St. Nicholas Avenue
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (1)
GPL20584 Illumina NextSeq 500 (Schizosaccharomyces pombe)
Samples (112)
GSM7847922 WT_Input rep1
GSM7847923 WT_BrdU rep1
GSM7847924 WT_H3K4me3_ChIP rep1
BioProject PRJNA996579

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Supplementary file Size Download File type/resource
GSE237770_RAW.tar 8.6 Gb (http)(custom) TAR (of BW, TAR)
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Raw data are available in SRA
Processed data provided as supplementary file

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