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Series GSE237637 Query DataSets for GSE237637
Status Public on May 30, 2024
Title The transcriptional landscape associated with the deletion of Regnase-1 [ATAC-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Having identified the crucial role of IFN-γ in anti-tumor immunity of Reg-1ΔNK, we attempted to elucidate the mechanism underlying the regulation of Ifng in the context of Regnase-1 deletion. Despite the notable increase in Ifng mRNA expression in Reg-1ΔNK-NK cells, Ifng was not a direct substrate of Regnase-1. However, the production of the IFN-γ was further enhanced upon stimulation with IL-12/18. We proposed that modifications may have occurred in the transcriptional regulation of Ifng in Reg-1ΔNK-NK cells.
 
Overall design To elucidate the transcriptional landscape associated with the deletion of Regnase-1, we performed paired assays for transposase-accessible chromatin sequencing (ATAC-seq) and RNA sequencing (RNA-seq) using Reg-1fl/fl- and Reg-1ΔNK-NK cells treated with or without IL-12/18.
 
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Submission date Jul 18, 2023
Last update date May 30, 2024
Contact name Xin Sun
E-mail(s) sunxin@ifrec.osaka-u.ac.jp
Organization name IFReC, Osaka University
Street address 3-1 Yamadaoka, Suita
City Osaka
ZIP/Postal code 5650871
Country Japan
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM7635033 WT_Ctl, rep 1, ATACseq
GSM7635034 WT_Ctl, rep 2, ATACseq
GSM7635035 WT_Ctl, rep 3, ATACseq
This SubSeries is part of SuperSeries:
GSE237643 Deletion of the mRNA endonuclease Regnase-1 promotes NK cell anti-tumor activity via OCT2- dependent transcription of Ifng
Relations
BioProject PRJNA996083

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE237637_RAW.tar 1.7 Gb (http)(custom) TAR (of BW, NARROWPEAK)
GSE237637_count.rds.gz 2.0 Mb (ftp)(http) RDS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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