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| Status |
Public on Dec 01, 2011 |
| Title |
Expression profiling of lung tissue from 6-, 10- and 14-week-old Fra2 transgenic male mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The transcription factor complex AP-1 (Activator protein 1) is composed of Jun (c-Jun, JunB, JunD) and Fos proteins (c-Fos, FosB, Fra-1, Fra-2) which control a variety of stress responses, including cell proliferation, apoptosis, inflammation, wound healing, and cancer. Individual Fos proteins have been thoroughly studied in gain- and loss-of-function mouse models, which revealed important functions in bone cell proliferation and differentiation. We have recently demonstrated that loss of Fra-2 causes perinatal lethality and severe osteopenia due to several cellular defects, including a chondrocyte differentiation defect and a control of osteoclast survival and size. Moreover, we have reported a profibrogenic function of Fra-2 in transgenic mice, in which ectopic expression of Fra-2 in various organs resulted in generalized fibrosis with predominant manifestations in the lung. Fra-2 knock-out newborns have increased numbers and size of osteoclasts in vivo. The pulmonary phenotype observed in Fra-2Tg mice is characterized by vascular remodeling and obliteration of pulmonary arteries, which coincides with expression of osteopontin, an AP-1 target gene involved in vascular remodeling and fibrogenesis. These alterations are followed by inflammation; release of profibrogenic factors, such as IL-4, insulin-like growth factor 1, and CXCL5.
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| Overall design |
The expression profiling study was performed to analyse changes in transcript levels in lung over a period of time.
Total RNA of four mutant male animals at each time point (age: 6, 10 and 14 weeks) were hybridised versus a pool of total RNA of four wild type mice of the corresponding age. For each mutant animal, two technical chip hybridisations were performed, including a dye-swap experiment (in total, 8 hybridisation of each time point = 2 technical replicates x 4 biological replicates).
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| Contributor(s) |
Beckers J, Horsch M, Schulz H, Götz A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Aug 23, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Martin Irmler |
| Organization name |
Helmholtz Zentrum München GmbH
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| Department |
Institute of Experimental Genetics
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| Lab |
Gene Regulation & Epigenetics
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| Street address |
Ingolstaedter Landstrasse 1
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| City |
Neuherberg |
| State/province |
Bayern |
| ZIP/Postal code |
85764 |
| Country |
Germany |
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| Platforms (1) |
| GPL4937 |
GSF/IEG mouse 21K array (+RZPD) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA130757 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23745_RAW.tar |
13.0 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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