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Series GSE23716 Query DataSets for GSE23716
Status Public on Nov 30, 2011
Title H3K27me3 is not required for recruitment of Polycomb repressor complex 1 to target loci in mouse embryonic stem cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The established hierarchical model explaining co-occupancy of Polycomb repressor complexes 1 and 2 (PRC 1 and 2) at target loci proposes that the chromodomain of the polycomb protein, a core PRC1 subunit, recognises the H3K27me3 histone modification catalysed by PRC2. We used chromatin immunoprecipitation to analyse PRC1 occupancy at target loci in Eed-/- mouse embryonic stem cells (ESCs) that lack H3K27me3. Occupancy of the core PRC1 proteins Ring1B and Mel18 was strongly reduced, consistent with the hierarchical model. However, levels of H2A ubiquitylation (H2AK119u1), the histone modification catalysed by PRC1, were similar to wild-type cells, suggesting PRC1 recruitment is independent of H3K27me3. ChIP-sequencing analysis of Ring1B occupancy genome wide substantiated this conclusion, demonstrating significant Ring1B levels at polycomb target loci in Eed-/- ESCs. Thus PRC1 and PRC2 are recruited independently to sites that they co-occupy. We conclude that the primary function of H3K27me3 is to increase the residency of PRC1 at target loci and thereby to contribute to the stability of PRC1 mediated silencing.
Overall design Examination of Ring1B binding in WT, Eed ko and Input of ESCs
Examination of CBX7 in WT and Eed ko of ESCs
Contributor(s) Tavares L, Brockdorff N
Citation(s) 22325148
Submission date Aug 19, 2010
Last update date May 15, 2019
Contact name Stephen Taylor
Phone +44 1865 222640
Organization name CBRG
Street address Headington
City Oxford
ZIP/Postal code OX3 9DS
Country United Kingdom
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (9)
GSM585229 EedWT-Ring1B
GSM585230 EedKO-Ring1B replicate 1
GSM585231 EedKO-Ring1B replicate 2
SRA SRP003786
BioProject PRJNA130883

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23716_Eed_KO.bam 2.0 Gb (ftp)(http) BAM
GSE23716_Eed_KO.gff3.gz 185.1 Kb (ftp)(http) GFF3
GSE23716_Eed_WT.gff3.gz 230.2 Kb (ftp)(http) GFF3
GSE23716_Input.bam 2.0 Gb (ftp)(http) BAM
GSE23716_RAW.tar 10.3 Gb (http)(custom) TAR (of BAM)
GSE23716_ST_Ring1B_Eed_WT_L5.bam 665.0 Mb (ftp)(http) BAM
SRA Run SelectorHelp
Processed data are available on Series record
Processed data provided as supplementary file
Raw data are available in SRA

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