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Series GSE237143 Query DataSets for GSE237143
Status Public on Oct 01, 2023
Title Single-cell transcriptome of stromal vascular fraction isolated from adipose tissue of lean and obese mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Visceral white adipose tissue (VAT) is susceptible to inflammation as a result of obesity, and this inflammation contributes to the development of obesity-related diseases. During the development of obesity, inflammatory immunocytes, including CD8+ T cells, Th1 cells, M1-like macrophages, neutrophils, and mast cells, accumulate in VAT. While these profound changes in the immunocyte composition of VAT lead to local inflammation, the triggers of VAT inflammation remain poorly understood. Therefore, a better understanding of the mechanisms underlying an early event of obesity-associated VAT inflammation will contribute to the development of novel therapies for obesity-related diseases. We utilized CellChat, which employs the expression of ligand-receptor pairs as indicators of intercellular communications, to examine the potential impact of these identified cell types within SVF on T cells. Our analysis revealed that ASCs exert the greatest influence on T cells in both lean and obese conditions. Finally, we demonstrated that ASCs promote Tcell infiltration into WAT during the early stages of obesity.
 
Overall design We performed single-cell sequencing (10X Genomics )of stromal vascular fraction from the epididymal white adipose tissue (eWAT) of normal chow diet (NCD)-fed and 12-week high fat diet (HFD)-fed mice.
 
Contributor(s) Deng T, Liao X, Zeng Q, Xie L
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Submission date Jul 12, 2023
Last update date Oct 02, 2023
Contact name Limin Xie
E-mail(s) xielimindr@gmail.com
Phone 13927573262
Organization name The second xiangya hospital of central south university
Street address No.139,Renmin Road Central Changsha, Hunan, China
City Changsha
State/province Hunan
ZIP/Postal code 410011
Country China
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM7596043 NCD; scRNAseq
GSM7596044 HFD; scRNAseq
Relations
BioProject PRJNA994061

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Supplementary file Size Download File type/resource
GSE237143_RAW.tar 60.6 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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